Molecular study of SHV-11 and SHV-12 genes among klebsiella pneumonia isolated from UTI patients in Erbil City

Abstract EN

Among 350 urine samples collected from urinary tract infection patients in Erbil city, (50) isolates were identified as Klebsiella pneumoniae using cultural, morphological and biochemical characteristics.

Their identity confirmed by Vitek2 system.

Extended-spectrum β-lactamase (ESBL) production determined by phenotypic and molecular methods.

Phenotypic detection methods include an initial screening by using (ATM30, CTX5 and CI30), by which (52%) were resistant.

Phenotypic ESBL production was confirmed by DDST and CDM.

Eight per cent of the isolates were ESBL producer by DDST and (42%) were ESBL producer by CDM.

Fifty per cent of the isolates were ESBL producer by both methods.

Polymerase Chain Reaction (PCR) amplification used for detection of SHV-11 (a non- ESBL) gene in the genomic DNA and SHV-12 (an ESBL) gene in the plasmid DNA.

SHV-11 found in the genomic DNA of 96% of ESBL producer isolates, while 88% of phenotypic ESBL negative isolates contained SHV-11 in their genomic DNA.

Seventy two per cent of the phenotypic ESBL producer isolates had SHV-12 in their plasmid DNA, whereas 44% of the phenotypic ESBL negative isolates contained SHV-12 in their plasmid DNA.

Out of 25 phenotypic ESBL producer isolates, 72% carried SHV-11 and SHV-12, whilst only 44% of phenotypic ESBL negative isolates contained both genes.