Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract for selective plant

العناوين الأخرى

نشاطية مستخلص الإيثيل أسيتات لنباتات مختارة و تنقيته كمضاد للميكروبات و الأكسدة

مقدم أطروحة جامعية

al-Mahadin, Saja Muhammad Salim

مشرف أطروحة جامعية

al-Zirini, Wail Ali Hasan

أعضاء اللجنة

Khulayfat, Khalid Muhammad
al-Mustafa, Ahmad Husayn Husayn
al-Nuaymat, Sulayman Muhammad

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2017

الملخص الإنجليزي

The increasing number of emerging new infections and antibiotic resistance in microorganisms entailed the search for new sources of bioactive natural products.

Medicinal plants, since ancient times, were considered as a safe and cheep medicinal alternative to treat various ailments.

Antimicrobial and antioxidant activities of ethyl acetate extract from aerial parts of Achillea fragrantissima were investigated.

The crude extract was subjected to different types of column chromatography (Silica gel and Sephadex LH-20) and purified compounds were isolated using TLC chromatography.

The resulting fractions and compounds were assessed for their antibacterial activity in agar diffusion test and minimum inhibitory concentration (MIC), as well as its antioxidant by DPPH assay.

Extract of A.

fragrantissima revealed antibacterial activity against Gram positive bacteria, namely, Bacillus subtilis.

The 1.5 mg/ml crude extract caused an inhibition zone of 13.0 mm to B.

subtilis with MIC of (400 μg/ml) for purified compounds .

The antioxidant activity of tested extract and purified fractions IC50 ranged from (11.87 to 52.59 μg/ml) and TEAC of (1.320 – 6.525 μg Trolox / μg plant extract).

The phytochemical screening indicate that purified compounds belong to aromatic hydrocarbons chemical groups.

Therefore, A.

fragrantissima represents a good candidate for isolation of antimicrobial compounds and antioxidant agents with pharmaceutical .

التخصصات الرئيسية

الأحياء

عدد الصفحات

33

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Design and methodology.

Chapter Four : Results, discussion and conclusion.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Mahadin, Saja Muhammad Salim. (2017). Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract for selective plant. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-780405

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Mahadin, Saja Muhammad Salim. Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract for selective plant. (Master's theses Theses and Dissertations Master). Mutah University. (2017).
https://search.emarefa.net/detail/BIM-780405

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Mahadin, Saja Muhammad Salim. (2017). Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract for selective plant. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-780405

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-780405