Assessment of the anticancer and antigenotoxic potential of costus speciosus extract against HCT116 cell line

الملخص EN

The aim of the present study was to analyze different effect of the costus speciosus extract against cancer cell line and to study its genotoxicity activity.

The whole plant extract was compare to most effective and known compound diosgnen.

For this purpose, antiproliferation and antigrowth activity was determined using cell viability assessment assays such as WST-1 assay and trypan blue dye exclusion staining assays against human colon tumor cell line (HCT116).

Acridine orange dye and Erythrosine-B dye was used to understand the mechanism, whether the death of cells is due to necrosis or Apoptosis.

Furthermore, Comet assay and Ames test were used for the detection of genetic toxicity of whole plant material.

Our results showed that with concentration of 300 μg/ml of whole plant extract almost showed 100 % of cell suppression in HCT116 cell line.

This data was also confirmed that the extract contained some compounds other than Diosgnen, which can suppress the growth of the cells.

Therefore, we might concluded that, the whole plant of costus speciosus contains active compounds other than Diosgnen, which has the ability to kill cancer cells