Assessment of the anticancer and antigenotoxic potential of costus speciosus extract against HCT116 cell line
Other Title(s)
تقييم قدرة نبات القسط المضادة للنمو السرطانى و التسمم الجيني باستخدام خلايا القولون السرطاني HCT116
Joint Authors
al-Thubayti, Iman Hilal
Umar, Abd al-Qadir Muhammad Shaykh
al-Matri, Muhammad Ali
al-Asuli, Sufyan Muhammad
Anwar, Yasir
Source
Journal of King Abdulaziz University : Sciences
Issue
Vol. 29, Issue 1 (30 Jun. 2017), pp.37-50, 14 p.
Publisher
King Abdulaziz University Scientific Publishing Center
Publication Date
2017-06-30
Country of Publication
Saudi Arabia
No. of Pages
14
Main Subjects
Abstract EN
The aim of the present study was to analyze different effect of the costus speciosus extract against cancer cell line and to study its genotoxicity activity.
The whole plant extract was compare to most effective and known compound diosgnen.
For this purpose, antiproliferation and antigrowth activity was determined using cell viability assessment assays such as WST-1 assay and trypan blue dye exclusion staining assays against human colon tumor cell line (HCT116).
Acridine orange dye and Erythrosine-B dye was used to understand the mechanism, whether the death of cells is due to necrosis or Apoptosis.
Furthermore, Comet assay and Ames test were used for the detection of genetic toxicity of whole plant material.
Our results showed that with concentration of 300 μg/ml of whole plant extract almost showed 100 % of cell suppression in HCT116 cell line.
This data was also confirmed that the extract contained some compounds other than Diosgnen, which can suppress the growth of the cells.
Therefore, we might concluded that, the whole plant of costus speciosus contains active compounds other than Diosgnen, which has the ability to kill cancer cells
American Psychological Association (APA)
al-Thubayti, Iman Hilal& Umar, Abd al-Qadir Muhammad Shaykh& al-Matri, Muhammad Ali& Anwar, Yasir& al-Asuli, Sufyan Muhammad. 2017. Assessment of the anticancer and antigenotoxic potential of costus speciosus extract against HCT116 cell line. Journal of King Abdulaziz University : Sciences،Vol. 29, no. 1, pp.37-50.
https://search.emarefa.net/detail/BIM-781671
Modern Language Association (MLA)
al-Thubayti, Iman Hilal…[et al.]. Assessment of the anticancer and antigenotoxic potential of costus speciosus extract against HCT116 cell line. Journal of King Abdulaziz University : Sciences Vol. 29, no. 1 (2017), pp.37-50.
https://search.emarefa.net/detail/BIM-781671
American Medical Association (AMA)
al-Thubayti, Iman Hilal& Umar, Abd al-Qadir Muhammad Shaykh& al-Matri, Muhammad Ali& Anwar, Yasir& al-Asuli, Sufyan Muhammad. Assessment of the anticancer and antigenotoxic potential of costus speciosus extract against HCT116 cell line. Journal of King Abdulaziz University : Sciences. 2017. Vol. 29, no. 1, pp.37-50.
https://search.emarefa.net/detail/BIM-781671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 49
Record ID
BIM-781671