Function of PD-L1 in antitumor immunity of glioma cells
المؤلفون المشاركون
Yongli, Lou
Jin, Shi
Dewei, Guo
Qurayshi, Ahmad Kalim
Laijun, Song
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 24، العدد 4 (31 مايو/أيار 2017)، ص ص. 803-807، 5ص.
الناشر
تاريخ النشر
2017-05-31
دولة النشر
السعودية
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
Human glioma is a highly fatal tumor with a significant feature of immune suppression.
The functions of PD-L1 refer to co-simulation and immune regulation.
To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro.
Expressions of PDL1mRNA and protein in the human glioma cell line were analyzed with quantitative RT-PCR and flow cytometer; and then expression of PD-L1 in tissue specimens of 10 glioma patients was treated with immunohistochemical analysis; glioma cell and allogeneic CD4+ and CD8+ T cells were co-cultured, and cytokine IFN-c, IL-2 and IL-10 in cultured supernatant fluid were determined with ELISA; upon blocking the interaction between glioma cell and the immune cell with PD-L1 monoclonal antibody (5H1), surface markers on immune cells were analyzed using flow cytometer.
All human glioma cell lines constitutively expressed PD-L1, and IFN-c induced glioma cell to highly express PD-L1.
It was shown through immunohistochemical analysis that glioma specimen expressed PD-L1, while expression of PD-L1 was not observed in normal tissue and normal human brain near the tumor location.
The release of IFN-c and IL-2 was inhibited, while IL-10 was increased slightly.
Glioma cell may escape from immune recognition and injury with the help of PD-L1, which is a significant pathogenic mechanism of glioma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yongli, Lou& Jin, Shi& Dewei, Guo& Qurayshi, Ahmad Kalim& Laijun, Song. 2017. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences،Vol. 24, no. 4, pp.803-807.
https://search.emarefa.net/detail/BIM-782063
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yongli, Lou…[et al.]. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences Vol. 24, no. 4 (May. 2017), pp.803-807.
https://search.emarefa.net/detail/BIM-782063
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yongli, Lou& Jin, Shi& Dewei, Guo& Qurayshi, Ahmad Kalim& Laijun, Song. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 4, pp.803-807.
https://search.emarefa.net/detail/BIM-782063
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 807
رقم السجل
BIM-782063
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر