Function of PD-L1 in antitumor immunity of glioma cells
Joint Authors
Yongli, Lou
Jin, Shi
Dewei, Guo
Qurayshi, Ahmad Kalim
Laijun, Song
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 4 (31 May. 2017), pp.803-807, 5 p.
Publisher
Publication Date
2017-05-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
Human glioma is a highly fatal tumor with a significant feature of immune suppression.
The functions of PD-L1 refer to co-simulation and immune regulation.
To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro.
Expressions of PDL1mRNA and protein in the human glioma cell line were analyzed with quantitative RT-PCR and flow cytometer; and then expression of PD-L1 in tissue specimens of 10 glioma patients was treated with immunohistochemical analysis; glioma cell and allogeneic CD4+ and CD8+ T cells were co-cultured, and cytokine IFN-c, IL-2 and IL-10 in cultured supernatant fluid were determined with ELISA; upon blocking the interaction between glioma cell and the immune cell with PD-L1 monoclonal antibody (5H1), surface markers on immune cells were analyzed using flow cytometer.
All human glioma cell lines constitutively expressed PD-L1, and IFN-c induced glioma cell to highly express PD-L1.
It was shown through immunohistochemical analysis that glioma specimen expressed PD-L1, while expression of PD-L1 was not observed in normal tissue and normal human brain near the tumor location.
The release of IFN-c and IL-2 was inhibited, while IL-10 was increased slightly.
Glioma cell may escape from immune recognition and injury with the help of PD-L1, which is a significant pathogenic mechanism of glioma.
American Psychological Association (APA)
Yongli, Lou& Jin, Shi& Dewei, Guo& Qurayshi, Ahmad Kalim& Laijun, Song. 2017. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences،Vol. 24, no. 4, pp.803-807.
https://search.emarefa.net/detail/BIM-782063
Modern Language Association (MLA)
Yongli, Lou…[et al.]. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences Vol. 24, no. 4 (May. 2017), pp.803-807.
https://search.emarefa.net/detail/BIM-782063
American Medical Association (AMA)
Yongli, Lou& Jin, Shi& Dewei, Guo& Qurayshi, Ahmad Kalim& Laijun, Song. Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 4, pp.803-807.
https://search.emarefa.net/detail/BIM-782063
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 807
Record ID
BIM-782063