Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter
المؤلفون المشاركون
Sadeghizadeh, Majid
Faghihloo, Ibrahim
Mokhtari Azad, Talat
Shahmahmudi, Shohreh
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 19، العدد 2 (28 فبراير/شباط 2017)، ص ص. 1-9، 9ص.
الناشر
تاريخ النشر
2017-02-28
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background: Cervical cancer is one of the most common cancers observed in women worldwide, and its development is related to E6 and E7 two viral oncoproteins of high-risk human papillomavirus (HPV) types.
Aberrant expression of E-cadherin, which is associated with epithelial-to-mesenchymal transition (EMT), is frequently observed in cervical cancer.
Objectives: The mechanisms underlying E-cadherin suppression in cervical cancer are not clear; therefore, this experimental study from Iran was designed to elucidate the relationship of DNA methyltransferase expression and E-cadherin promoter methylation with E-cadherin expression in HPV-16 E6- and E7-expressing cells.
Materials and Methods: Real-time PCR and western blot were used to determine the effects of HPV-16 E6 and E7 on E-cadherin, DNMT1, DNMT3a, and DNMT3b expression in HCT-116 cell line.We also analyzed E-cadherin promoter methylation in cells expressing HPV-16 E6 and E7 oncoproteins by bisulfite sequencing.
Results: HPV-16 E6 and E7 proteins reduced E-cadherin expression 3.7 and 2.2 timeswhencompared with control cells (P = 0.0221 and P = 0.0461, respectively).
This reduction was greater in HPV-16 E6-expressing cells than in HPV-16 E7-expressing cells.
Although HPV-16 E6 and E7 increased DNA methyltransferase 1 expression 2.6 and 3.4 times, respectively (P = 0.0133 and P = 0.0113) when compared with control cells, they was no E-cadherin promoter methylation.
Conclusions: Unlike other cancer-associated viruses (HBV, HCV, and EBV), reduction in E-cadherin expression in HPV-16 E6- and E7- expressing cells is not due to hypermethylation of the E-cadherin promoter.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faghihloo, Ibrahim& Sadeghizadeh, Majid& Shahmahmudi, Shohreh& Mokhtari Azad, Talat. 2017. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal،Vol. 19, no. 2, pp.1-9.
https://search.emarefa.net/detail/BIM-791797
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faghihloo, Ibrahim…[et al.]. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal Vol. 19, no. 2 (Feb. 2017), pp.1-9.
https://search.emarefa.net/detail/BIM-791797
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faghihloo, Ibrahim& Sadeghizadeh, Majid& Shahmahmudi, Shohreh& Mokhtari Azad, Talat. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal. 2017. Vol. 19, no. 2, pp.1-9.
https://search.emarefa.net/detail/BIM-791797
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8-9
رقم السجل
BIM-791797
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر