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Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter
Joint Authors
Sadeghizadeh, Majid
Faghihloo, Ibrahim
Mokhtari Azad, Talat
Shahmahmudi, Shohreh
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 19, Issue 2 (28 Feb. 2017), pp.1-9, 9 p.
Publisher
Publication Date
2017-02-28
Country of Publication
United Arab Emirates
No. of Pages
9
Main Subjects
Abstract EN
Background: Cervical cancer is one of the most common cancers observed in women worldwide, and its development is related to E6 and E7 two viral oncoproteins of high-risk human papillomavirus (HPV) types.
Aberrant expression of E-cadherin, which is associated with epithelial-to-mesenchymal transition (EMT), is frequently observed in cervical cancer.
Objectives: The mechanisms underlying E-cadherin suppression in cervical cancer are not clear; therefore, this experimental study from Iran was designed to elucidate the relationship of DNA methyltransferase expression and E-cadherin promoter methylation with E-cadherin expression in HPV-16 E6- and E7-expressing cells.
Materials and Methods: Real-time PCR and western blot were used to determine the effects of HPV-16 E6 and E7 on E-cadherin, DNMT1, DNMT3a, and DNMT3b expression in HCT-116 cell line.We also analyzed E-cadherin promoter methylation in cells expressing HPV-16 E6 and E7 oncoproteins by bisulfite sequencing.
Results: HPV-16 E6 and E7 proteins reduced E-cadherin expression 3.7 and 2.2 timeswhencompared with control cells (P = 0.0221 and P = 0.0461, respectively).
This reduction was greater in HPV-16 E6-expressing cells than in HPV-16 E7-expressing cells.
Although HPV-16 E6 and E7 increased DNA methyltransferase 1 expression 2.6 and 3.4 times, respectively (P = 0.0133 and P = 0.0113) when compared with control cells, they was no E-cadherin promoter methylation.
Conclusions: Unlike other cancer-associated viruses (HBV, HCV, and EBV), reduction in E-cadherin expression in HPV-16 E6- and E7- expressing cells is not due to hypermethylation of the E-cadherin promoter.
American Psychological Association (APA)
Faghihloo, Ibrahim& Sadeghizadeh, Majid& Shahmahmudi, Shohreh& Mokhtari Azad, Talat. 2017. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal،Vol. 19, no. 2, pp.1-9.
https://search.emarefa.net/detail/BIM-791797
Modern Language Association (MLA)
Faghihloo, Ibrahim…[et al.]. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal Vol. 19, no. 2 (Feb. 2017), pp.1-9.
https://search.emarefa.net/detail/BIM-791797
American Medical Association (AMA)
Faghihloo, Ibrahim& Sadeghizadeh, Majid& Shahmahmudi, Shohreh& Mokhtari Azad, Talat. Transcriptional suppression of E-cadherin by HPV-16 E6 and E7 oncogenes is independent of hypermethylation of E-cadherin promoter. Iranian Red Crescent Medical Journal. 2017. Vol. 19, no. 2, pp.1-9.
https://search.emarefa.net/detail/BIM-791797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-9
Record ID
BIM-791797