Genetic diversity, variation in essential oil content and antibacterial activity among different populations of Juniperus Phoenicea L. from Jordan

العناوين الأخرى

التنوع الوراثي الكيميائي لنبات العرعر الفينيقي في الأردن و فعالية الزيوت المستخلصة منه ضد بعض الجراثيم

مقدم أطروحة جامعية

al-Khalayifah, Inas Muhammad

مشرف أطروحة جامعية

al-Khulayfat, Khalid Muhammad Awf

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2012

الملخص الإنجليزي

Juniperus phoenicea L.

tree (Araâr) is a wild species and characterized by a medicinal value.

Juniperus phoenicea is found predominantly in the southern heights of Jordan.

Due to the threat that may minimize this 3species biodiversity, recently there has been considerable attention paid in understanding its importance and how biodiversity supports ecosystem processes.

Thus the genetic and chemical profiles were very much taken into consideration during characterization the biodiversity statues of the J.

phoenicea.

In the present study, J.

phoenicea samples from five natural populations in Southern Jordan were collected and their genetic variations were studied.

In addition, the hydro-distilled essential oils from three geographical regions were characterized for its chemical composition and antibacterial activity.

The genetic variation among and within the selected populations was evaluated by mean of inter simple sequence repeats (ISSR).

The analysis of the ISSR data for the two groups showed that a small but significant amount of genetic variations (0.47% of the total) and larger; significant amount (5.3% of the total) are due to differences between regions and variations between individuals within populations, respectively.

GC and GC-MS analysis of the chemical constituents of the isolated oils mainly revealed the presence of monoterpene hydrocarbons, sesquiterpene hydrocarbons, oxygenated monoterpenes, and oxygenated sesquiterpenes.

Monoterpene hydrocarbons represent the major constituent in most studied samples among of which α-pinene is the main compound.

This study revealed the occurrence of variation in yield and chemical composition of the studied populations according to their geographical origin, extracted part and genetic influence.

The essential oils exhibited wide range of antibacterial activities (weak to excellent) as measured by hole plate diffusion and MIC assay.

A correlation between the variation in chemical composition and antibacterial activity of the principal oils was observed, and the recorded variation in the antibacterial activity found to be significant.

التخصصات الرئيسية

الأحياء

عدد الصفحات

102

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-793331