Genetic diversity, variation in essential oil content and antibacterial activity among different populations of Juniperus Phoenicea L. from Jordan
Other Title(s)
التنوع الوراثي الكيميائي لنبات العرعر الفينيقي في الأردن و فعالية الزيوت المستخلصة منه ضد بعض الجراثيم
Dissertant
Thesis advisor
al-Khulayfat, Khalid Muhammad Awf
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2012
English Abstract
Juniperus phoenicea L.
tree (Araâr) is a wild species and characterized by a medicinal value.
Juniperus phoenicea is found predominantly in the southern heights of Jordan.
Due to the threat that may minimize this 3species biodiversity, recently there has been considerable attention paid in understanding its importance and how biodiversity supports ecosystem processes.
Thus the genetic and chemical profiles were very much taken into consideration during characterization the biodiversity statues of the J.
phoenicea.
In the present study, J.
phoenicea samples from five natural populations in Southern Jordan were collected and their genetic variations were studied.
In addition, the hydro-distilled essential oils from three geographical regions were characterized for its chemical composition and antibacterial activity.
The genetic variation among and within the selected populations was evaluated by mean of inter simple sequence repeats (ISSR).
The analysis of the ISSR data for the two groups showed that a small but significant amount of genetic variations (0.47% of the total) and larger; significant amount (5.3% of the total) are due to differences between regions and variations between individuals within populations, respectively.
GC and GC-MS analysis of the chemical constituents of the isolated oils mainly revealed the presence of monoterpene hydrocarbons, sesquiterpene hydrocarbons, oxygenated monoterpenes, and oxygenated sesquiterpenes.
Monoterpene hydrocarbons represent the major constituent in most studied samples among of which α-pinene is the main compound.
This study revealed the occurrence of variation in yield and chemical composition of the studied populations according to their geographical origin, extracted part and genetic influence.
The essential oils exhibited wide range of antibacterial activities (weak to excellent) as measured by hole plate diffusion and MIC assay.
A correlation between the variation in chemical composition and antibacterial activity of the principal oils was observed, and the recorded variation in the antibacterial activity found to be significant.
Main Subjects
No. of Pages
102
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Review of literature.
Chapter Three : Material and methods.
Chapter Four : Results.
Chapter Five : Discussion and conclusion and recommendation.
References.
American Psychological Association (APA)
al-Khalayifah, Inas Muhammad. (2012). Genetic diversity, variation in essential oil content and antibacterial activity among different populations of Juniperus Phoenicea L. from Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-793331
Modern Language Association (MLA)
al-Khalayifah, Inas Muhammad. Genetic diversity, variation in essential oil content and antibacterial activity among different populations of Juniperus Phoenicea L. from Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2012).
https://search.emarefa.net/detail/BIM-793331
American Medical Association (AMA)
al-Khalayifah, Inas Muhammad. (2012). Genetic diversity, variation in essential oil content and antibacterial activity among different populations of Juniperus Phoenicea L. from Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-793331
Language
English
Data Type
Arab Theses
Record ID
BIM-793331
