Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study
المؤلفون المشاركون
Ali, Inam N.
Awad, Muthanna M.
Mahmud, Ala S.
المصدر
Yemeni Journal for Medical Sciences
العدد
المجلد 11، العدد 1 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2017-12-31
دولة النشر
اليمن
عدد الصفحات
7
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
Objective: This study aimed to assess the effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and activities of some enzymatic antioxidants.
Methods: Fifty-six mature male Wistar rats were divided into two experimental groups and a control group.
The first experimental group was sub-divided into three sub-groups depending on concentration of methotrexate (MTX): X1 (0.05 mg/kg MTX), X2 (0.125 mg/kg MTX) and X3 (0.250 mg/kg MTX), which were given intraperitoneally on a weekly basis for eight weeks.
The second experimental group (MTX and omega-3 group) was also sub-divided into three sub-groups (Y1, Y2 and Y3), which were injected intraperitoneally with 0.05, 0.125 and 0.25 mg/kg MTX, respectively, weekly for eight weeks accompanied by the oral administration of 300 mg/kg omega-3..
The rats of the control group were given distilled water.
The enzymatic activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured in the sera of rats.
In addition, the mitotic index (MI) and chromosomal aberrations of bone marrow were also studied.
Results: MTX resulted in a significant decrease in the activities of CAT, SOD and GR compared to the controls.
It also led to an increase in the MI and chromosomal aberrations of rat bone marrows.
On the other hand, omega-3 significantly increased the activities of the investigated enzymatic antioxidants and reduced the MI and chromosomal aberrations in treated mice when given in combination with MTX.
Conclusions: MTX has a genotoxic effect on the bone marrow by increasing the MI and all types of chromosomal aberrations and decreases the enzymatic activity of CAT, SOD and GR.
The addition of omega-3 leads to a protective effect by reducing the toxic and mutagenic effects of MTX.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ali, Inam N.& Awad, Muthanna M.& Mahmud, Ala S.. 2017. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences،Vol. 11, no. 1, pp.1-7.
https://search.emarefa.net/detail/BIM-813279
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ali, Inam N.…[et al.]. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences Vol. 11, no. 1 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-813279
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ali, Inam N.& Awad, Muthanna M.& Mahmud, Ala S.. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences. 2017. Vol. 11, no. 1, pp.1-7.
https://search.emarefa.net/detail/BIM-813279
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-813279
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر