Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study
Joint Authors
Ali, Inam N.
Awad, Muthanna M.
Mahmud, Ala S.
Source
Yemeni Journal for Medical Sciences
Issue
Vol. 11, Issue 1 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
University of Science and Technology
Publication Date
2017-12-31
Country of Publication
Yemen
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Objective: This study aimed to assess the effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and activities of some enzymatic antioxidants.
Methods: Fifty-six mature male Wistar rats were divided into two experimental groups and a control group.
The first experimental group was sub-divided into three sub-groups depending on concentration of methotrexate (MTX): X1 (0.05 mg/kg MTX), X2 (0.125 mg/kg MTX) and X3 (0.250 mg/kg MTX), which were given intraperitoneally on a weekly basis for eight weeks.
The second experimental group (MTX and omega-3 group) was also sub-divided into three sub-groups (Y1, Y2 and Y3), which were injected intraperitoneally with 0.05, 0.125 and 0.25 mg/kg MTX, respectively, weekly for eight weeks accompanied by the oral administration of 300 mg/kg omega-3..
The rats of the control group were given distilled water.
The enzymatic activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured in the sera of rats.
In addition, the mitotic index (MI) and chromosomal aberrations of bone marrow were also studied.
Results: MTX resulted in a significant decrease in the activities of CAT, SOD and GR compared to the controls.
It also led to an increase in the MI and chromosomal aberrations of rat bone marrows.
On the other hand, omega-3 significantly increased the activities of the investigated enzymatic antioxidants and reduced the MI and chromosomal aberrations in treated mice when given in combination with MTX.
Conclusions: MTX has a genotoxic effect on the bone marrow by increasing the MI and all types of chromosomal aberrations and decreases the enzymatic activity of CAT, SOD and GR.
The addition of omega-3 leads to a protective effect by reducing the toxic and mutagenic effects of MTX.
American Psychological Association (APA)
Ali, Inam N.& Awad, Muthanna M.& Mahmud, Ala S.. 2017. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences،Vol. 11, no. 1, pp.1-7.
https://search.emarefa.net/detail/BIM-813279
Modern Language Association (MLA)
Ali, Inam N.…[et al.]. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences Vol. 11, no. 1 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-813279
American Medical Association (AMA)
Ali, Inam N.& Awad, Muthanna M.& Mahmud, Ala S.. Effect of methotrexate and omega-3 combination on cytogenetic changes of bone marrow and some enzymatic antioxidants : an experimental study. Yemeni Journal for Medical Sciences. 2017. Vol. 11, no. 1, pp.1-7.
https://search.emarefa.net/detail/BIM-813279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-7
Record ID
BIM-813279