Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq
العناوين الأخرى
سيادة جينات المضخة و جينات الحماية الكروموسومية بين أنواع جرثومة الايرومونس المقاومة للتتراسايكلين المعزولة من مرضى الإسهال في العراق
المؤلفون المشاركون
al-Jarakh, Ala Hani
Abd al-Muttalib, Lubna
المصدر
al-Qadisiyah Journal of Veterinary Medicine Sciences
العدد
المجلد 15، العدد 1 (30 يونيو/حزيران 2016)، ص ص. 101-108، 8ص.
الناشر
جامعة القادسية كلية الطب البيطري
تاريخ النشر
2016-06-30
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The study aim to investigate the prevalence of tetracycline resistant Aeromonas hydrophila isolated from clinical sources in Hilla city - Iraq, using polymerase chain reaction (PCR) technique.
A total of 822 samples were collected from fecal specimens from patients.
Samples were collected from rectal swab (routine work) and from those who suffering from diarrhea.
Isolates were identified using cultural and biochemical tests to the level of species and then identification was confirmed using viteks 2 system and molecular technique using 16S rRNA specific primer.
PCR was used to detect tetracycline resistance isolates.
Out of 822 clinical samples collected, 13 isolates (1.58%) were belonged to Aeromonas spp.
However, other bacterial isolates belonged to other genera similar to Aeromonas were also recovered.
Out of 13 Aeromonas spp., eight A.
hydrophila isolates (61.53%) were obtained, while the other isolates were distributed as: four isolates of A.
salmoncidia (30.76%), and one of A.
sobria.
Isolation and detection of A.
salmoncidia species was first recorded in Iraq.
Results found that all tetracycline-resistant isolates carried at least one of the tet genes examined.
Among efflux genes, tet(A) was the most commonly observed in isolates (No.8), found in 6 isolates (75%), followed by Efflux tet gene (tet B,C) (62.5%) and chromosomal protection protein (tetO) 5 (62.5%).
Tet (E) efflux gene was found in two isolates (25%).
Efflux pump (tetL) and chromosomal protect gene tet (M) was detected in one isolates (12.5%).
However efflux genes tet (D and G) were not detected in any of the isolates.
The prevalence of tet O chromosomal protection protein among A.
hydrophila isolates was first recorded in Iraq and in many other countries.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. 2016. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 1, pp.101-108.
https://search.emarefa.net/detail/BIM-819116
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 1 (2016), pp.101-108.
https://search.emarefa.net/detail/BIM-819116
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 1, pp.101-108.
https://search.emarefa.net/detail/BIM-819116
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 107-108
رقم السجل
BIM-819116
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر