Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq
Other Title(s)
سيادة جينات المضخة و جينات الحماية الكروموسومية بين أنواع جرثومة الايرومونس المقاومة للتتراسايكلين المعزولة من مرضى الإسهال في العراق
Joint Authors
al-Jarakh, Ala Hani
Abd al-Muttalib, Lubna
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.101-108, 8 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
The study aim to investigate the prevalence of tetracycline resistant Aeromonas hydrophila isolated from clinical sources in Hilla city - Iraq, using polymerase chain reaction (PCR) technique.
A total of 822 samples were collected from fecal specimens from patients.
Samples were collected from rectal swab (routine work) and from those who suffering from diarrhea.
Isolates were identified using cultural and biochemical tests to the level of species and then identification was confirmed using viteks 2 system and molecular technique using 16S rRNA specific primer.
PCR was used to detect tetracycline resistance isolates.
Out of 822 clinical samples collected, 13 isolates (1.58%) were belonged to Aeromonas spp.
However, other bacterial isolates belonged to other genera similar to Aeromonas were also recovered.
Out of 13 Aeromonas spp., eight A.
hydrophila isolates (61.53%) were obtained, while the other isolates were distributed as: four isolates of A.
salmoncidia (30.76%), and one of A.
sobria.
Isolation and detection of A.
salmoncidia species was first recorded in Iraq.
Results found that all tetracycline-resistant isolates carried at least one of the tet genes examined.
Among efflux genes, tet(A) was the most commonly observed in isolates (No.8), found in 6 isolates (75%), followed by Efflux tet gene (tet B,C) (62.5%) and chromosomal protection protein (tetO) 5 (62.5%).
Tet (E) efflux gene was found in two isolates (25%).
Efflux pump (tetL) and chromosomal protect gene tet (M) was detected in one isolates (12.5%).
However efflux genes tet (D and G) were not detected in any of the isolates.
The prevalence of tet O chromosomal protection protein among A.
hydrophila isolates was first recorded in Iraq and in many other countries.
American Psychological Association (APA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. 2016. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 1, pp.101-108.
https://search.emarefa.net/detail/BIM-819116
Modern Language Association (MLA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 1 (2016), pp.101-108.
https://search.emarefa.net/detail/BIM-819116
American Medical Association (AMA)
Abd al-Muttalib, Lubna& al-Jarakh, Ala Hani. Prevalence of efflux genes and chromosomal protection protein genes among tetracycline resistant Aeromonas spp. isolated from diarrheic patients in Iraq. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 1, pp.101-108.
https://search.emarefa.net/detail/BIM-819116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 107-108
Record ID
BIM-819116