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Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state
المؤلفون المشاركون
Mukhtar, Ala Abd Allah
Abd al-Fadil, Nuha Ahmad
Muhammad, Malik Sulayman
al-Zaki, Salah al-Din G.
Hasan, Muhammad Salih
al-Tayyib, Hisham Nur al-Dayim
المصدر
Sudan Journal of Medical Sciences
العدد
المجلد 13، العدد 3 (30 سبتمبر/أيلول 2018)، ص ص. 175-186، 12ص.
الناشر
جامعة أم درمان الإسلامية كلية الطب
تاريخ النشر
2018-09-30
دولة النشر
السودان
عدد الصفحات
12
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
Background: The presence of pathogenic bacteria in circulated currency was recorded as a public health hazard.
In this study, all examined Sudanese banknotes (100%) were found to be contaminated by gram-negative bacteria.
Proteus mirabilis were recovered from 10 examined notes (22.2%, f = 10), E.
coli (13.3%, f = 6) and Klebsiella spp.
(8.9%, f = 4) were also identified.
Only the most resistant P.
mirabilis isolate was identified using culture-based and 16S rRNA gene sequencing techniques.
Methods: Proteus isolates were identified phenotypically and tested for their susceptibility to 16 of commonly used antibiotics, then most resistant isolate was confirmed genotypically via 16S rRNA gene amplification and sequencing.
Bioinformatics analysis using BLAST for sequence similarity search, Clustal W program for multiple sequence alignment, MEGA7 software for phylogenetic analysis.
Tree was constructed to show the evolutionary relationships of the obtained sequence with similar sequences in the databases using.
Results: The obtained sequence was found to be 100% identical to P.
mirabilis 16S rRNA gene using BLAST.
The phylogenetic tree was constructed to show the evolutionary relationships of the obtained sequence with similar sequences in the databases using MEGA7 software, and the closest strain was found to be P.
mirabilis strain from India (EU411047).
Conclusion: This study has shown that some currency notes circulated at Khartoum transportation are carriers of antimicrobial-resistant P.
mirabilis that could be potential source for their transmission in public.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mukhtar, Ala Abd Allah& Abd al-Fadil, Nuha Ahmad& Muhammad, Malik Sulayman& al-Tayyib, Hisham Nur al-Dayim& al-Zaki, Salah al-Din G.& Hasan, Muhammad Salih. 2018. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences،Vol. 13, no. 3, pp.175-186.
https://search.emarefa.net/detail/BIM-835499
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mukhtar, Ala Abd Allah…[et al.]. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences Vol. 13, no. 3 (2018), pp.175-186.
https://search.emarefa.net/detail/BIM-835499
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mukhtar, Ala Abd Allah& Abd al-Fadil, Nuha Ahmad& Muhammad, Malik Sulayman& al-Tayyib, Hisham Nur al-Dayim& al-Zaki, Salah al-Din G.& Hasan, Muhammad Salih. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences. 2018. Vol. 13, no. 3, pp.175-186.
https://search.emarefa.net/detail/BIM-835499
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 183-186
رقم السجل
BIM-835499
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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