Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state
Joint Authors
Mukhtar, Ala Abd Allah
Abd al-Fadil, Nuha Ahmad
Muhammad, Malik Sulayman
al-Zaki, Salah al-Din G.
Hasan, Muhammad Salih
al-Tayyib, Hisham Nur al-Dayim
Source
Sudan Journal of Medical Sciences
Issue
Vol. 13, Issue 3 (30 Sep. 2018), pp.175-186, 12 p.
Publisher
Omdurman Islamic University Faculty of Medicine
Publication Date
2018-09-30
Country of Publication
Sudan
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Background: The presence of pathogenic bacteria in circulated currency was recorded as a public health hazard.
In this study, all examined Sudanese banknotes (100%) were found to be contaminated by gram-negative bacteria.
Proteus mirabilis were recovered from 10 examined notes (22.2%, f = 10), E.
coli (13.3%, f = 6) and Klebsiella spp.
(8.9%, f = 4) were also identified.
Only the most resistant P.
mirabilis isolate was identified using culture-based and 16S rRNA gene sequencing techniques.
Methods: Proteus isolates were identified phenotypically and tested for their susceptibility to 16 of commonly used antibiotics, then most resistant isolate was confirmed genotypically via 16S rRNA gene amplification and sequencing.
Bioinformatics analysis using BLAST for sequence similarity search, Clustal W program for multiple sequence alignment, MEGA7 software for phylogenetic analysis.
Tree was constructed to show the evolutionary relationships of the obtained sequence with similar sequences in the databases using.
Results: The obtained sequence was found to be 100% identical to P.
mirabilis 16S rRNA gene using BLAST.
The phylogenetic tree was constructed to show the evolutionary relationships of the obtained sequence with similar sequences in the databases using MEGA7 software, and the closest strain was found to be P.
mirabilis strain from India (EU411047).
Conclusion: This study has shown that some currency notes circulated at Khartoum transportation are carriers of antimicrobial-resistant P.
mirabilis that could be potential source for their transmission in public.
American Psychological Association (APA)
Mukhtar, Ala Abd Allah& Abd al-Fadil, Nuha Ahmad& Muhammad, Malik Sulayman& al-Tayyib, Hisham Nur al-Dayim& al-Zaki, Salah al-Din G.& Hasan, Muhammad Salih. 2018. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences،Vol. 13, no. 3, pp.175-186.
https://search.emarefa.net/detail/BIM-835499
Modern Language Association (MLA)
Mukhtar, Ala Abd Allah…[et al.]. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences Vol. 13, no. 3 (2018), pp.175-186.
https://search.emarefa.net/detail/BIM-835499
American Medical Association (AMA)
Mukhtar, Ala Abd Allah& Abd al-Fadil, Nuha Ahmad& Muhammad, Malik Sulayman& al-Tayyib, Hisham Nur al-Dayim& al-Zaki, Salah al-Din G.& Hasan, Muhammad Salih. Identification of Proteus mirabilis on banknotes using 16s rRNA Gene in Khartoum state. Sudan Journal of Medical Sciences. 2018. Vol. 13, no. 3, pp.175-186.
https://search.emarefa.net/detail/BIM-835499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 183-186
Record ID
BIM-835499