Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway
المؤلفون المشاركون
Zheng, Guanlin
Li, Haizhen
Yang, Libo
Yao, Shutong
Chen, Shihong
Zheng, Maochuan
Tian, Hua
Zhang, Tie
Zhao, Qin
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 5 (31 يوليو/تموز 2018)، ص ص. 849-857، 9ص.
الناشر
تاريخ النشر
2018-07-31
دولة النشر
السعودية
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Irisin is a newly discovered myokine which can relieve metabolic disorders and resist atherosclerosis.
The effects of irisin on ox-LDL-induced macrophage apoptosis and endoplasmic reticulum stress-related pathways were observed in vitro.
RAW264.7 macrophages were cultured in vitro and pretreated with irisin at 20, 40 and 80 ng/ml for 30 min, followed by culture with 100 mg/L ox-LDL and 5 mg/L tunicamycin (TM) for 12 h.
The cell viability and apoptosis were detected by MTT assay and annexin V-FITC double staining.
The nuclear translocation of activating transcription factor 6 (ATF6) was detected by immunofluorescence assay.
Western blot was used to detect the expressions of p-PERK, p-eIF2a, C/EBP homologous protein (CHOP) and Bcl-2.
Irisin reduced lipid accumulation in macrophages in a concentration-dependent pattern and significantly inhibited apoptosis induced by ox-LDL and TM.
Compared with ox-LDL and TM groups, the expressions of CHOP, p-PERK and p-eIF2a in the irisin group significantly decreased, the translocation of ATF6 from cytoplasm to nucleus was significantly weakened, and Bcl-2 expression significantly increased.
Irisin can alleviate the apoptosis of macrophages induced by ox-LDL, which may be achieved by inhibiting the PERK/eIF2a/CHOP and ATF6/CHOP endoplasmic reticulum stress signaling pathways
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Haizhen& Zhang, Tie& Yang, Libo& Yao, Shutong& Chen, Shihong& Zheng, Maochuan…[et al.]. 2018. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences،Vol. 25, no. 5, pp.849-857.
https://search.emarefa.net/detail/BIM-838460
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Haizhen…[et al.]. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences Vol. 25, no. 5 (Jul. 2018), pp.849-857.
https://search.emarefa.net/detail/BIM-838460
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Haizhen& Zhang, Tie& Yang, Libo& Yao, Shutong& Chen, Shihong& Zheng, Maochuan…[et al.]. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 5, pp.849-857.
https://search.emarefa.net/detail/BIM-838460
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 856-857
رقم السجل
BIM-838460
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر