Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway
Joint Authors
Zheng, Guanlin
Li, Haizhen
Yang, Libo
Yao, Shutong
Chen, Shihong
Zheng, Maochuan
Tian, Hua
Zhang, Tie
Zhao, Qin
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 5 (31 Jul. 2018), pp.849-857, 9 p.
Publisher
Publication Date
2018-07-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Abstract EN
Irisin is a newly discovered myokine which can relieve metabolic disorders and resist atherosclerosis.
The effects of irisin on ox-LDL-induced macrophage apoptosis and endoplasmic reticulum stress-related pathways were observed in vitro.
RAW264.7 macrophages were cultured in vitro and pretreated with irisin at 20, 40 and 80 ng/ml for 30 min, followed by culture with 100 mg/L ox-LDL and 5 mg/L tunicamycin (TM) for 12 h.
The cell viability and apoptosis were detected by MTT assay and annexin V-FITC double staining.
The nuclear translocation of activating transcription factor 6 (ATF6) was detected by immunofluorescence assay.
Western blot was used to detect the expressions of p-PERK, p-eIF2a, C/EBP homologous protein (CHOP) and Bcl-2.
Irisin reduced lipid accumulation in macrophages in a concentration-dependent pattern and significantly inhibited apoptosis induced by ox-LDL and TM.
Compared with ox-LDL and TM groups, the expressions of CHOP, p-PERK and p-eIF2a in the irisin group significantly decreased, the translocation of ATF6 from cytoplasm to nucleus was significantly weakened, and Bcl-2 expression significantly increased.
Irisin can alleviate the apoptosis of macrophages induced by ox-LDL, which may be achieved by inhibiting the PERK/eIF2a/CHOP and ATF6/CHOP endoplasmic reticulum stress signaling pathways
American Psychological Association (APA)
Li, Haizhen& Zhang, Tie& Yang, Libo& Yao, Shutong& Chen, Shihong& Zheng, Maochuan…[et al.]. 2018. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences،Vol. 25, no. 5, pp.849-857.
https://search.emarefa.net/detail/BIM-838460
Modern Language Association (MLA)
Li, Haizhen…[et al.]. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences Vol. 25, no. 5 (Jul. 2018), pp.849-857.
https://search.emarefa.net/detail/BIM-838460
American Medical Association (AMA)
Li, Haizhen& Zhang, Tie& Yang, Libo& Yao, Shutong& Chen, Shihong& Zheng, Maochuan…[et al.]. Irisin protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting the endoplasmic reticulum stress pathway. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 5, pp.849-857.
https://search.emarefa.net/detail/BIM-838460
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 856-857
Record ID
BIM-838460