Chromium inhibits in vitro viability and steroidogenic in ram leydig cells

الملخص EN

One of an ecological hazardous agent Chromium; Cr (VI), The present experiment in vitro design to examine the mechanism of Leydig cell functions of Cr (VI) in ram testis, Cr (VI) treated groups demising cell growth behavior of exponential phase was upset of feeding time to Leydig cell in a dose-dependent manner, and induced mitochondria-dependent ATP depletion and subsequently apoptosis.

Cr (VI) effect may be attributed, at least in part to DNA fragmentation increase DNA tail number and tail length of COMET as compared with control group.

Furthermore, the properties of cell-specific regulation of cell membrane integrity had reduced and determinant cell concentration drop an.d reflected on the testosterone concentration were decreased as concentration-dependent manner, In conclusion, our results display the Cr (VI) is cytotoxic and impairs both viability and steroidogenic functions of Leydig cells in ram testis via actually different pathway direct affecting of viability and indirect on steroidogenic activity, succeeding in testicular performance.

However, the definite modes of action of harmfulness are not evidently unknown and must be rechecked and studied in a different aspect.