Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions
المؤلفون المشاركون
Ahmadi zadeh, Changiz
Mirzaei, Hamid
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 20، العدد 3 (31 مارس/آذار 2018)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2018-03-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background: Lactobacilli are commonly used organisms in the production of probiotics.
Objectives: The present study was conducted to examine the effect of probiotics isolated from dairy products on the expression of Stx1 and Stx2 genes in verotoxigenic E.
coli (VTEC).
Methods: This experimental study was conducted during April and January 2016 in East Azerbaijan, Iran.
At first, Hundred samples of traditional dairy products were collected.
Then, using standard phenotypic and genotypic methods, the Lactobacillus casei (L.
casei) and lactobacillus (L.
plantarum) were studied.
Subsequently, these strains were examined along with the pathogenic bacteria strain of Escherichia coli (E.
coli) (O157: H7) by neighboring cultivation.
After extraction of mRNA, the expression of these genes was determined using real- time PCR.
Results: The results revealed that L.
casei reduces the expression of Shiga toxin (Stx1 and Stx2) genes more compared to L.
plantarum (P = 0.001).
Moreover, it was found that both Lactobacilli reduce the expression of Stx1 genes more than Stx2.
Conclusions: Probiotics could be used to prevent and control intestinal diseases caused by E.
coli.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. 2018. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal،Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal Vol. 20, no. 3 (Mar. 2018), pp.1-7.
https://search.emarefa.net/detail/BIM-840312
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-840312
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر