Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec)‎ in laboratory conditions

المؤلفون المشاركون

Ahmadi zadeh, Changiz
Mirzaei, Hamid

المصدر

Iranian Red Crescent Medical Journal

العدد

المجلد 20، العدد 3 (31 مارس/آذار 2018)، ص ص. 1-7، 7ص.

الناشر

المستشفى الإيراني

تاريخ النشر

2018-03-31

دولة النشر

الإمارات العربية المتحدة

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

Background: Lactobacilli are commonly used organisms in the production of probiotics.

Objectives: The present study was conducted to examine the effect of probiotics isolated from dairy products on the expression of Stx1 and Stx2 genes in verotoxigenic E.

coli (VTEC).

Methods: This experimental study was conducted during April and January 2016 in East Azerbaijan, Iran.

At first, Hundred samples of traditional dairy products were collected.

Then, using standard phenotypic and genotypic methods, the Lactobacillus casei (L.

casei) and lactobacillus (L.

plantarum) were studied.

Subsequently, these strains were examined along with the pathogenic bacteria strain of Escherichia coli (E.

coli) (O157: H7) by neighboring cultivation.

After extraction of mRNA, the expression of these genes was determined using real- time PCR.

Results: The results revealed that L.

casei reduces the expression of Shiga toxin (Stx1 and Stx2) genes more compared to L.

plantarum (P = 0.001).

Moreover, it was found that both Lactobacilli reduce the expression of Stx1 genes more than Stx2.

Conclusions: Probiotics could be used to prevent and control intestinal diseases caused by E.

coli.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Ahmadi zadeh, Changiz& Mirzaei, Hamid. 2018. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal،Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal Vol. 20, no. 3 (Mar. 2018), pp.1-7.
https://search.emarefa.net/detail/BIM-840312

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 6-7

رقم السجل

BIM-840312