Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions
Joint Authors
Ahmadi zadeh, Changiz
Mirzaei, Hamid
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 20, Issue 3 (31 Mar. 2018), pp.1-7, 7 p.
Publisher
Publication Date
2018-03-31
Country of Publication
United Arab Emirates
No. of Pages
7
Main Subjects
Abstract EN
Background: Lactobacilli are commonly used organisms in the production of probiotics.
Objectives: The present study was conducted to examine the effect of probiotics isolated from dairy products on the expression of Stx1 and Stx2 genes in verotoxigenic E.
coli (VTEC).
Methods: This experimental study was conducted during April and January 2016 in East Azerbaijan, Iran.
At first, Hundred samples of traditional dairy products were collected.
Then, using standard phenotypic and genotypic methods, the Lactobacillus casei (L.
casei) and lactobacillus (L.
plantarum) were studied.
Subsequently, these strains were examined along with the pathogenic bacteria strain of Escherichia coli (E.
coli) (O157: H7) by neighboring cultivation.
After extraction of mRNA, the expression of these genes was determined using real- time PCR.
Results: The results revealed that L.
casei reduces the expression of Shiga toxin (Stx1 and Stx2) genes more compared to L.
plantarum (P = 0.001).
Moreover, it was found that both Lactobacilli reduce the expression of Stx1 genes more than Stx2.
Conclusions: Probiotics could be used to prevent and control intestinal diseases caused by E.
coli.
American Psychological Association (APA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. 2018. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal،Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312
Modern Language Association (MLA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal Vol. 20, no. 3 (Mar. 2018), pp.1-7.
https://search.emarefa.net/detail/BIM-840312
American Medical Association (AMA)
Ahmadi zadeh, Changiz& Mirzaei, Hamid. Evaluation of the effect of probiotics isolated from traditional dairy products on the expression of stx1 and stx2 genes of verotoxigenic escherichia coli (vtec) in laboratory conditions. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-840312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-7
Record ID
BIM-840312