Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells
المؤلفون المشاركون
al-Dasuqi, Wail
Ubayd, Narin Hafiz
al-Shirshabi, Hanan Muhammad
المصدر
Arab Journal of Nuclear Sciences and Applications
العدد
المجلد 51، العدد 3 (31 يوليو/تموز 2018)، ص ص. 139-151، 13ص.
الناشر
الجمعية المصرية للعلوم النووية و تطبيقاتها
تاريخ النشر
2018-07-31
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Purpose: The purpose of this work is to study the effect of green tea and grape seed extracts mixture (GTE-GSE mix) and γ-irradiation or their combination to enhance the antiproliferative effect on the growth of myeloma cells.
Method: Myeloma cell line SP2/OR was cultured in RPMI-1640 medium; total count and viability % were daily calculated.
Myeloma cells (1 X 106/ml) were treated with GTE-GSE mix concentrations (100 - 1000 ng/ml), γ-irradiation doses (0.5 - 10 Gy) and their combination for 6 days.
Evaluation of Caspase 8 and 9 activities were measured.
Cell culture media and GTE-GSE mix activity was determined by DPPH method.
Results: The most effective dose of GTE-GSE mix on myeloma cells growth inhibition and viability % was 1000 ng/ml, while the effective dose of γ-irradiation was 8 Gy where viability percentage was zero.
The obtained results indicated that treatment with GTE-GSE mix plus γ-irradiation combination (700 ng/ml + 6 Gy) resulted to zero% growth inhibition at 4th day.
Treatment with combination dose induced activation of caspase 8 and caspase 9 levels more than GTE-GSE mix and γ-irradiation alone.
Treatment with GTE-GSE mix and γ-irradiation individually resulted in significant reduction in DPPH activity to 62% while in combination treatment was 73.5%.
Conclusion: Treatment with GTE-GSE mix plus γ-irradiation (700 ng/ml + 6 Gy) combination resulted in marked increase of growth inhibition levels, apoptosis and cancer cells regression when compared with each agent alone.
GTE-GSE mix plus γ-irradiation combination had an antiproliferative effect on the growth of myeloma cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shirshabi, Hanan Muhammad& al-Dasuqi, Wail& Ubayd, Narin Hafiz. 2018. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications،Vol. 51, no. 3, pp.139-151.
https://search.emarefa.net/detail/BIM-880560
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shirshabi, Hanan Muhammad…[et al.]. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications Vol. 51, no. 3 (Jul. 2018), pp.139-151.
https://search.emarefa.net/detail/BIM-880560
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shirshabi, Hanan Muhammad& al-Dasuqi, Wail& Ubayd, Narin Hafiz. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications. 2018. Vol. 51, no. 3, pp.139-151.
https://search.emarefa.net/detail/BIM-880560
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 148-151
رقم السجل
BIM-880560
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر