Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells
Joint Authors
al-Dasuqi, Wail
Ubayd, Narin Hafiz
al-Shirshabi, Hanan Muhammad
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 51, Issue 3 (31 Jul. 2018), pp.139-151, 13 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2018-07-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Purpose: The purpose of this work is to study the effect of green tea and grape seed extracts mixture (GTE-GSE mix) and γ-irradiation or their combination to enhance the antiproliferative effect on the growth of myeloma cells.
Method: Myeloma cell line SP2/OR was cultured in RPMI-1640 medium; total count and viability % were daily calculated.
Myeloma cells (1 X 106/ml) were treated with GTE-GSE mix concentrations (100 - 1000 ng/ml), γ-irradiation doses (0.5 - 10 Gy) and their combination for 6 days.
Evaluation of Caspase 8 and 9 activities were measured.
Cell culture media and GTE-GSE mix activity was determined by DPPH method.
Results: The most effective dose of GTE-GSE mix on myeloma cells growth inhibition and viability % was 1000 ng/ml, while the effective dose of γ-irradiation was 8 Gy where viability percentage was zero.
The obtained results indicated that treatment with GTE-GSE mix plus γ-irradiation combination (700 ng/ml + 6 Gy) resulted to zero% growth inhibition at 4th day.
Treatment with combination dose induced activation of caspase 8 and caspase 9 levels more than GTE-GSE mix and γ-irradiation alone.
Treatment with GTE-GSE mix and γ-irradiation individually resulted in significant reduction in DPPH activity to 62% while in combination treatment was 73.5%.
Conclusion: Treatment with GTE-GSE mix plus γ-irradiation (700 ng/ml + 6 Gy) combination resulted in marked increase of growth inhibition levels, apoptosis and cancer cells regression when compared with each agent alone.
GTE-GSE mix plus γ-irradiation combination had an antiproliferative effect on the growth of myeloma cells.
American Psychological Association (APA)
al-Shirshabi, Hanan Muhammad& al-Dasuqi, Wail& Ubayd, Narin Hafiz. 2018. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications،Vol. 51, no. 3, pp.139-151.
https://search.emarefa.net/detail/BIM-880560
Modern Language Association (MLA)
al-Shirshabi, Hanan Muhammad…[et al.]. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications Vol. 51, no. 3 (Jul. 2018), pp.139-151.
https://search.emarefa.net/detail/BIM-880560
American Medical Association (AMA)
al-Shirshabi, Hanan Muhammad& al-Dasuqi, Wail& Ubayd, Narin Hafiz. Antiproliferative effect of green tea and grape seed mixture extracts combined with γ-irradiation on the growth of myeloma cells. Arab Journal of Nuclear Sciences and Applications. 2018. Vol. 51, no. 3, pp.139-151.
https://search.emarefa.net/detail/BIM-880560
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 148-151
Record ID
BIM-880560