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L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells
المؤلفون المشاركون
Khan, Umran Ali
Fatimah, Nuzhah
Khan, Muhammad Mazhar al-Din
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 6 (30 سبتمبر/أيلول 2019)، ص ص. 1146-1153، 8ص.
الناشر
تاريخ النشر
2019-09-30
دولة النشر
السعودية
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age.
L-asparaginase is an important therapeutic enzyme used for treating ALL.
Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale.
Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase.
However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients.
Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme.
In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase.
Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay.
The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL.
These results indicate that pseudomonal L-asparaginase may be used for cancer treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fatimah, Nuzhah& Khan, Muhammad Mazhar al-Din& Khan, Umran Ali. 2019. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fatimah, Nuzhah…[et al.]. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fatimah, Nuzhah& Khan, Muhammad Mazhar al-Din& Khan, Umran Ali. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1153
رقم السجل
BIM-889188
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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