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L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells
Joint Authors
Khan, Umran Ali
Fatimah, Nuzhah
Khan, Muhammad Mazhar al-Din
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 6 (30 Sep. 2019), pp.1146-1153, 8 p.
Publisher
Publication Date
2019-09-30
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
Abstract EN
Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age.
L-asparaginase is an important therapeutic enzyme used for treating ALL.
Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale.
Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase.
However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients.
Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme.
In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase.
Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay.
The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL.
These results indicate that pseudomonal L-asparaginase may be used for cancer treatment.
American Psychological Association (APA)
Fatimah, Nuzhah& Khan, Muhammad Mazhar al-Din& Khan, Umran Ali. 2019. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
Modern Language Association (MLA)
Fatimah, Nuzhah…[et al.]. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
American Medical Association (AMA)
Fatimah, Nuzhah& Khan, Muhammad Mazhar al-Din& Khan, Umran Ali. L-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1146-1153.
https://search.emarefa.net/detail/BIM-889188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1153
Record ID
BIM-889188