Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa
المؤلفون المشاركون
Khalifah, Ashraf Yasin Zaki
al-Maliki, M.
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 6 (30 سبتمبر/أيلول 2019)، ص ص. 1262-1267، 6ص.
الناشر
تاريخ النشر
2019-09-30
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
In this study, one bacterial strain, ESM-1, was isolated from rhizosphere of Eruca sativa, growing in Al Hofouf, Saudia Arabia, after enrichment with methanol as a sole carbon and energy source in a batch culture.
ESM-1 was characterized by a polyphasic approach.
The strain was identified as Delftia acidovorans at similarity level of 99.9% of the 16S rRNA gene sequences.
Results of the Biolog Gen III MicroPlate test system showed that strain ESM-1 reacted positively to 47 (50%) including the one-carbon compound formic acid, and partially positive to 6 (∼6.4%) out of the 94 different the traits examined.
The total cellular fatty acids composition of the strain ESM-1 was (C16:1ω7c/C16:1ω6c) and C16:0) and matched that of Delftia acidovorans at a similarity index of 0.9, providing a robustness to the ESM-1 identification.
Furthermore, ESM-1 displayed a complex polar lipid profile consisting of phosphatidylethanolamine, phosphatidylglycerol, glycolipid, aminolipid, in addition to uncharacterized lipids.
The DNA G+C content of the strain was 66.6 mol%.
Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain ESM1-1 was clearly clustered within the Delftia clade and constructed a monophyletic subcluster with Delftia acidovorans NBRC14950.
The results addressed that ESM-1 is a facultative methylotrophic bacterium indigenous to Al Hofouf region and opens the door for potential biotechnological applications (e.g., bioremediation) of this strain, in future.
Additionally, these findings assure that the total cellular fatty acid analysis and 16S rRNA gene are reliable tool for bacterial characterization and identification.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. 2019. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1266-1267
رقم السجل
BIM-889201
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر