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Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa
Joint Authors
Khalifah, Ashraf Yasin Zaki
al-Maliki, M.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 6 (30 Sep. 2019), pp.1262-1267, 6 p.
Publisher
Publication Date
2019-09-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
In this study, one bacterial strain, ESM-1, was isolated from rhizosphere of Eruca sativa, growing in Al Hofouf, Saudia Arabia, after enrichment with methanol as a sole carbon and energy source in a batch culture.
ESM-1 was characterized by a polyphasic approach.
The strain was identified as Delftia acidovorans at similarity level of 99.9% of the 16S rRNA gene sequences.
Results of the Biolog Gen III MicroPlate test system showed that strain ESM-1 reacted positively to 47 (50%) including the one-carbon compound formic acid, and partially positive to 6 (∼6.4%) out of the 94 different the traits examined.
The total cellular fatty acids composition of the strain ESM-1 was (C16:1ω7c/C16:1ω6c) and C16:0) and matched that of Delftia acidovorans at a similarity index of 0.9, providing a robustness to the ESM-1 identification.
Furthermore, ESM-1 displayed a complex polar lipid profile consisting of phosphatidylethanolamine, phosphatidylglycerol, glycolipid, aminolipid, in addition to uncharacterized lipids.
The DNA G+C content of the strain was 66.6 mol%.
Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain ESM1-1 was clearly clustered within the Delftia clade and constructed a monophyletic subcluster with Delftia acidovorans NBRC14950.
The results addressed that ESM-1 is a facultative methylotrophic bacterium indigenous to Al Hofouf region and opens the door for potential biotechnological applications (e.g., bioremediation) of this strain, in future.
Additionally, these findings assure that the total cellular fatty acid analysis and 16S rRNA gene are reliable tool for bacterial characterization and identification.
American Psychological Association (APA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. 2019. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
Modern Language Association (MLA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
American Medical Association (AMA)
Khalifah, Ashraf Yasin Zaki& al-Maliki, M.. Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1262-1267.
https://search.emarefa.net/detail/BIM-889201
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1266-1267
Record ID
BIM-889201