Bioethanol production with carboxymethylcellulase of Pseudomonas poae using castor bean (Ricinus communis L.)‎ cake

الملخص EN

Increased consumption of fossil fuels is an emerging problem.

Scientists look for the existence of other alternatives to fossil fuels, including so-called renewable energy.

Accordingly, we report the production of bio-ethanol from the remnants of castor oil bean seed cake (CBC) by the carboxymethylcellulase enzyme (CMCase).

A bacterial strain isolated from rice straw showing higher CMCase activity was identified.

The 16S rRNA result showed a 93 % homology with the 16SrRNA gene sequences of Pseudomonas poae RE ⁄ 1-1-14, the strain was identified as Pseudomonas poae AB3.

In addition, our results showed that the highest enzyme activity was achieved after 48 h and inoculum size of 3.7 _ 105 CFU.

The optimum temperature, pH and Carboxymethylcellulose (CMC) concentration for the highest enzyme activity was 25 _C, pH 7 and 10 g/l respectively.

Furthermore, The CMCase was purified by ammonium sulphate at a concentration of 60 %.

The SDS-PAGE of the purified enzyme showed a molecular weight of 88 kDa.

Additionally, the (CBC) was hydrolyzed by the purified CMCase at the enzyme optimum conditions.

The results showed the liberation of 5.2 g / L of reducing sugar by using dinitrosalicylic acid (DNS) assay.

Finally, the total sugar produces 35 g / L after 48 h when Saccharomyces cerevisiae was used as a fermentation agent.

Hence for the first time, we have been successfully able to produce bioethanol from CBC with CMCase of Pseudomonas poae.