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Bioethanol production with carboxymethylcellulase of Pseudomonas poae using castor bean (Ricinus communis L.) cake
Joint Authors
Ubadah, Imad
al-Fifi, Zaraq Isa Ali
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 4 (31 May. 2019), pp.866-871, 6 p.
Publisher
Publication Date
2019-05-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Increased consumption of fossil fuels is an emerging problem.
Scientists look for the existence of other alternatives to fossil fuels, including so-called renewable energy.
Accordingly, we report the production of bio-ethanol from the remnants of castor oil bean seed cake (CBC) by the carboxymethylcellulase enzyme (CMCase).
A bacterial strain isolated from rice straw showing higher CMCase activity was identified.
The 16S rRNA result showed a 93 % homology with the 16SrRNA gene sequences of Pseudomonas poae RE ⁄ 1-1-14, the strain was identified as Pseudomonas poae AB3.
In addition, our results showed that the highest enzyme activity was achieved after 48 h and inoculum size of 3.7 _ 105 CFU.
The optimum temperature, pH and Carboxymethylcellulose (CMC) concentration for the highest enzyme activity was 25 _C, pH 7 and 10 g/l respectively.
Furthermore, The CMCase was purified by ammonium sulphate at a concentration of 60 %.
The SDS-PAGE of the purified enzyme showed a molecular weight of 88 kDa.
Additionally, the (CBC) was hydrolyzed by the purified CMCase at the enzyme optimum conditions.
The results showed the liberation of 5.2 g / L of reducing sugar by using dinitrosalicylic acid (DNS) assay.
Finally, the total sugar produces 35 g / L after 48 h when Saccharomyces cerevisiae was used as a fermentation agent.
Hence for the first time, we have been successfully able to produce bioethanol from CBC with CMCase of Pseudomonas poae.
American Psychological Association (APA)
Ubadah, Imad& al-Fifi, Zaraq Isa Ali& Uthman, Muhammad E.. 2019. Bioethanol production with carboxymethylcellulase of Pseudomonas poae using castor bean (Ricinus communis L.) cake. Saudi Journal of Biological Sciences،Vol. 26, no. 4, pp.866-871.
https://search.emarefa.net/detail/BIM-891178
Modern Language Association (MLA)
Uthman, Muhammad E.…[et al.]. Bioethanol production with carboxymethylcellulase of Pseudomonas poae using castor bean (Ricinus communis L.) cake. Saudi Journal of Biological Sciences Vol. 26, no. 4 (May. 2019), pp.866-871.
https://search.emarefa.net/detail/BIM-891178
American Medical Association (AMA)
Ubadah, Imad& al-Fifi, Zaraq Isa Ali& Uthman, Muhammad E.. Bioethanol production with carboxymethylcellulase of Pseudomonas poae using castor bean (Ricinus communis L.) cake. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 4, pp.866-871.
https://search.emarefa.net/detail/BIM-891178
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 871
Record ID
BIM-891178