Evaluation of femA gene and different primers for mecA gene for detection of methicillin-resistant Staphylococcus aureus (MRSA)‎

الملخص EN

The clinical specimens (wound and burn swab, abscess and pus, urine and blood culture) were collected from 122 patients admitted to different hospitals in Erbil city/Iraq.

Depending on cultural, morphological and biochemical characteristics 38 isolates were identified as S.

aureus.

Antibiotic sensitivity testing was done for all isolates by using 8 antibiotics.

Vancomycin was the most effective antibiotics against Staphylococcus aureus while only (31.57%) isolates were resistant to it.

While Penicillin and Methicillin were the least effects when (94.87%) and (86.84%) isolates were resistant to them respectively.

Through phenotypic detection for Methicillin-resistant S.

aureus (MRSA) among the 38 isolated S.

aureus, 28 (73.68%) isolates were detected as MRSA and 10 (26.31%) isolates were detected as Methicillin-sensitive S.

aureus (MSSA).

Among S.

aureus isolates, only 16 (42.10%) gave a positive result for mecA gene detection by PCR when used mecA 1st set primers, while 18 (47.36%) positive result for mecA 2nd set primers.

Otherwise, all 38 (100%) S.

aureus isolates showed a positive result for femA gene by PCR method