Evaluation of femA gene and different primers for mecA gene for detection of methicillin-resistant Staphylococcus aureus (MRSA)
Joint Authors
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 31, Issue 3 (30 Jun. 2019), pp.16-22, 7 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
The clinical specimens (wound and burn swab, abscess and pus, urine and blood culture) were collected from 122 patients admitted to different hospitals in Erbil city/Iraq.
Depending on cultural, morphological and biochemical characteristics 38 isolates were identified as S.
aureus.
Antibiotic sensitivity testing was done for all isolates by using 8 antibiotics.
Vancomycin was the most effective antibiotics against Staphylococcus aureus while only (31.57%) isolates were resistant to it.
While Penicillin and Methicillin were the least effects when (94.87%) and (86.84%) isolates were resistant to them respectively.
Through phenotypic detection for Methicillin-resistant S.
aureus (MRSA) among the 38 isolated S.
aureus, 28 (73.68%) isolates were detected as MRSA and 10 (26.31%) isolates were detected as Methicillin-sensitive S.
aureus (MSSA).
Among S.
aureus isolates, only 16 (42.10%) gave a positive result for mecA gene detection by PCR when used mecA 1st set primers, while 18 (47.36%) positive result for mecA 2nd set primers.
Otherwise, all 38 (100%) S.
aureus isolates showed a positive result for femA gene by PCR method
American Psychological Association (APA)
Amin, Bushra K.& Nasir, Nada. 2019. Evaluation of femA gene and different primers for mecA gene for detection of methicillin-resistant Staphylococcus aureus (MRSA). ZANCO Journal of Pure and Applied Sciences،Vol. 31, no. 3, pp.16-22.
https://search.emarefa.net/detail/BIM-892357
Modern Language Association (MLA)
Amin, Bushra K.& Nasir, Nada. Evaluation of femA gene and different primers for mecA gene for detection of methicillin-resistant Staphylococcus aureus (MRSA). ZANCO Journal of Pure and Applied Sciences Vol. 31, no. 3 (2019), pp.16-22.
https://search.emarefa.net/detail/BIM-892357
American Medical Association (AMA)
Amin, Bushra K.& Nasir, Nada. Evaluation of femA gene and different primers for mecA gene for detection of methicillin-resistant Staphylococcus aureus (MRSA). ZANCO Journal of Pure and Applied Sciences. 2019. Vol. 31, no. 3, pp.16-22.
https://search.emarefa.net/detail/BIM-892357
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 20-22
Record ID
BIM-892357