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Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor
العناوين الأخرى
كفاءة أنزيم البولي فينول أوكسيديز المقيد في تفكيك بعض صبغات الأنسجة باستخدام نظام الوجبة في المفاعل الحيوي
المؤلف
المصدر
The Iraqi Journal of Agricultural Science
العدد
المجلد 50، العدد 3 (30 يونيو/حزيران 2019)، ص ص. 943-950، 8ص.
الناشر
تاريخ النشر
2019-06-30
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
In the current study, three types of common plants, namely Tomato (Solanum lycopersicum), Cucumber (Cucumis sativus) and Orange (Citrus sinensis) were obtained and screened for their polyphenol oxidase (PPO) activity, Among the three plants, Solanum lycopersicum was chosen with maximum enzymatic activity, it had the highest productivity of the enzyme (23733 U/mg protein).The PPO from Solanum lycopersicum was purified using two steps: concentration by sucrose and gel filtration by using Sephacryl S-200.
The results showed an increase in the final purification folds by 2.4 times with an enzyme yield of 32.6%.
The immobilization studies showed that PPO was more stable when immobilized on chitosan by covalent linkage with immobilization ratio of 62%, in comparison with agar-agar by entrapment method (36%).
The removal efficiency of crude and partial purified PPO was studied with textile dyes, including yellow, red, black and blue dyes at optimum conditions: pH 5, temperature 40oC after 3 hrs.
Maximum removal efficiency of dyes observed with crude PPO were 53.9, 81.4, 86.5 and 79.6% respectively.
However, purified PPO displayed removal efficiency reached 60.3, 84.3, 84.6 and 77.5% respectively.
The potential of immobilized PPO on chitosan was evaluated by decolorization of black textile dye in packed bed bioreactor in batch operation.
The results indicated that immobilized PPO in batch operation has the ability to remove 99% of the dye after 2 hrs, and the results showed a positive relationship between the degradation rate and incubation time in batch operation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayn, ٍSahar Irhaim. 2019. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science،Vol. 50, no. 3, pp.943-950.
https://search.emarefa.net/detail/BIM-893024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayn, ٍSahar Irhaim. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science Vol. 50, no. 3 (2019), pp.943-950.
https://search.emarefa.net/detail/BIM-893024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayn, ٍSahar Irhaim. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 3, pp.943-950.
https://search.emarefa.net/detail/BIM-893024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 949-950
رقم السجل
BIM-893024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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