Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor
Other Title(s)
كفاءة أنزيم البولي فينول أوكسيديز المقيد في تفكيك بعض صبغات الأنسجة باستخدام نظام الوجبة في المفاعل الحيوي
Author
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 3 (30 Jun. 2019), pp.943-950, 8 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
In the current study, three types of common plants, namely Tomato (Solanum lycopersicum), Cucumber (Cucumis sativus) and Orange (Citrus sinensis) were obtained and screened for their polyphenol oxidase (PPO) activity, Among the three plants, Solanum lycopersicum was chosen with maximum enzymatic activity, it had the highest productivity of the enzyme (23733 U/mg protein).The PPO from Solanum lycopersicum was purified using two steps: concentration by sucrose and gel filtration by using Sephacryl S-200.
The results showed an increase in the final purification folds by 2.4 times with an enzyme yield of 32.6%.
The immobilization studies showed that PPO was more stable when immobilized on chitosan by covalent linkage with immobilization ratio of 62%, in comparison with agar-agar by entrapment method (36%).
The removal efficiency of crude and partial purified PPO was studied with textile dyes, including yellow, red, black and blue dyes at optimum conditions: pH 5, temperature 40oC after 3 hrs.
Maximum removal efficiency of dyes observed with crude PPO were 53.9, 81.4, 86.5 and 79.6% respectively.
However, purified PPO displayed removal efficiency reached 60.3, 84.3, 84.6 and 77.5% respectively.
The potential of immobilized PPO on chitosan was evaluated by decolorization of black textile dye in packed bed bioreactor in batch operation.
The results indicated that immobilized PPO in batch operation has the ability to remove 99% of the dye after 2 hrs, and the results showed a positive relationship between the degradation rate and incubation time in batch operation.
American Psychological Association (APA)
Husayn, ٍSahar Irhaim. 2019. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science،Vol. 50, no. 3, pp.943-950.
https://search.emarefa.net/detail/BIM-893024
Modern Language Association (MLA)
Husayn, ٍSahar Irhaim. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science Vol. 50, no. 3 (2019), pp.943-950.
https://search.emarefa.net/detail/BIM-893024
American Medical Association (AMA)
Husayn, ٍSahar Irhaim. Effeciency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 3, pp.943-950.
https://search.emarefa.net/detail/BIM-893024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 949-950
Record ID
BIM-893024