Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases
المؤلفون المشاركون
Abd al-Ghaffar, Muhammad M.
al-Subki, Muna M.
Harba, Nansi M.
Radi, Amani A.
Abu Khalil, Nuha A.
المصدر
Journal of the Egyptian Society of Parasitology
العدد
المجلد 49، العدد 2 (31 أغسطس/آب 2019)، ص ص. 265-276، 12ص.
الناشر
الجمعية المصرية لعلم الطفيليات
تاريخ النشر
2019-08-31
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Acanthamoeba genus is a medically important free living amoeba causing serious humans infections.
Amoebic keratitis (AK) is a sight threatening infection of cornea caused by Acanthamoeba pathogenic genotypes, which prevalence remarkably increased in developed countries.
The study compared different methods for diagnosing AK and identified Acanthamoeba genotypes by molecular examination in contact lens wearers (CLWs).
Patients were 79 clinical corneal swaps (CS) and 15 samples from contact lens storage cases (CLSC).
Clinical CSs were divided into four groups; GI: 20 patients suffering from chronic corneal ulcers, GII: 15 patients with traumatic ulcers, GIII: 24symptomatic CLWs and GIV: 20asymptomatic control individuals.
CLSC were provided from apparently healthy asymptomatic CLWs (15).
Swabs and solution samples were underwent microscopic and staining examination, cultivation on non-nutrient agar (NNA) plates and PCR molecular analysis.
Sequencing and genotyping of PCR- positive samples were performed.
The results showed that Acanthamoeba parasites were detected in 3.8% of CS and 6.7% of CLSC samples.
The highest significantly positive results were by culture (3.8%) followed by Giemsa and trichrome stains (2.5%) and lastly direct microscopy (1.3%) of CS samples.
Only one positive sample (6.7%) was detected in CLSC by all methods, but without statistical significance.
Sensitivity of PCR compared to culture was 25%.
Acanthamoeba parasites in CS were from subgroup II with 12.5% detection rate in CLWs, but the positive case from CLSC was from subgroup I with 6.7% detection rate.
This study confirmed different risk factors in association with AK in CLWs.
Genotype determination for Acanthamoeba positive case by PCR revealed homology with Acanthamoeba genotype T9 isolate ICS20
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Ghaffar, Muhammad M.& al-Subki, Muna M.& Harba, Nansi M.& Radi, Amani A.& Abu Khalil, Nuha A.. 2019. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology،Vol. 49, no. 2, pp.265-276.
https://search.emarefa.net/detail/BIM-893250
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Ghaffar, Muhammad M.…[et al.]. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology Vol. 49, no. 2 (Aug. 2019), pp.265-276.
https://search.emarefa.net/detail/BIM-893250
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Ghaffar, Muhammad M.& al-Subki, Muna M.& Harba, Nansi M.& Radi, Amani A.& Abu Khalil, Nuha A.. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology. 2019. Vol. 49, no. 2, pp.265-276.
https://search.emarefa.net/detail/BIM-893250
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes appendix : p. 275-276
رقم السجل
BIM-893250
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر