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Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases
Joint Authors
Abd al-Ghaffar, Muhammad M.
al-Subki, Muna M.
Harba, Nansi M.
Radi, Amani A.
Abu Khalil, Nuha A.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 49, Issue 2 (31 Aug. 2019), pp.265-276, 12 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2019-08-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Acanthamoeba genus is a medically important free living amoeba causing serious humans infections.
Amoebic keratitis (AK) is a sight threatening infection of cornea caused by Acanthamoeba pathogenic genotypes, which prevalence remarkably increased in developed countries.
The study compared different methods for diagnosing AK and identified Acanthamoeba genotypes by molecular examination in contact lens wearers (CLWs).
Patients were 79 clinical corneal swaps (CS) and 15 samples from contact lens storage cases (CLSC).
Clinical CSs were divided into four groups; GI: 20 patients suffering from chronic corneal ulcers, GII: 15 patients with traumatic ulcers, GIII: 24symptomatic CLWs and GIV: 20asymptomatic control individuals.
CLSC were provided from apparently healthy asymptomatic CLWs (15).
Swabs and solution samples were underwent microscopic and staining examination, cultivation on non-nutrient agar (NNA) plates and PCR molecular analysis.
Sequencing and genotyping of PCR- positive samples were performed.
The results showed that Acanthamoeba parasites were detected in 3.8% of CS and 6.7% of CLSC samples.
The highest significantly positive results were by culture (3.8%) followed by Giemsa and trichrome stains (2.5%) and lastly direct microscopy (1.3%) of CS samples.
Only one positive sample (6.7%) was detected in CLSC by all methods, but without statistical significance.
Sensitivity of PCR compared to culture was 25%.
Acanthamoeba parasites in CS were from subgroup II with 12.5% detection rate in CLWs, but the positive case from CLSC was from subgroup I with 6.7% detection rate.
This study confirmed different risk factors in association with AK in CLWs.
Genotype determination for Acanthamoeba positive case by PCR revealed homology with Acanthamoeba genotype T9 isolate ICS20
American Psychological Association (APA)
Abd al-Ghaffar, Muhammad M.& al-Subki, Muna M.& Harba, Nansi M.& Radi, Amani A.& Abu Khalil, Nuha A.. 2019. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology،Vol. 49, no. 2, pp.265-276.
https://search.emarefa.net/detail/BIM-893250
Modern Language Association (MLA)
Abd al-Ghaffar, Muhammad M.…[et al.]. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology Vol. 49, no. 2 (Aug. 2019), pp.265-276.
https://search.emarefa.net/detail/BIM-893250
American Medical Association (AMA)
Abd al-Ghaffar, Muhammad M.& al-Subki, Muna M.& Harba, Nansi M.& Radi, Amani A.& Abu Khalil, Nuha A.. Comparison between different methods for diagnosis of Acanthamoeba infection and isolation of genotype T9 from a contact lens cases. Journal of the Egyptian Society of Parasitology. 2019. Vol. 49, no. 2, pp.265-276.
https://search.emarefa.net/detail/BIM-893250
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 275-276
Record ID
BIM-893250