Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells
المؤلفون المشاركون
Nashiri, Muhammad
al-Khatib, Alya
Froemming, Gabriele
المصدر
Yemeni Journal for Medical Sciences
العدد
المجلد 12، العدد 1 (31 ديسمبر/كانون الأول 2018)، ص ص. 37-43، 7ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
اليمن
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
الملخص EN
Objectives: The aim of the current study was to investigate the effects of berberine chloride and various heat conditions on the gene expression of Osterix, RUNX-2, RANKL, CDK2, CDK4, IL-6 and IL-11.
Methods: Six groups of cells were treated with hyperthermia for 1 h: Two groups at mild, two at moderate and two at severe hyperthermia (39, 43 and 45°C respectively).
Berberine chloride (80 µg/mL) was selected for treating one group of mild, moderate and severe hyperthermia (combination).
All treated groups were recovered at 37°C for 24 h.
Another exposure for hyperthermia (1 h) and recovery for 3 h at 37°C were applied.
Results: The expression of Osterix was highly upregulated in all groups except in the severe hyperthermia and mild hyperthermia with berberine groups.
Only the mild hyperthermia without berberine induced a slight increase in the expression of RUNX2, whereas severe hyperthermia alone suppressed the levels on a significant manner.
Berberine alone was more effective in significantly up-regulating RANKL expression.
On the other hand, CDK2 mRNA was downregulated in all groups.
CDK4 showed a similar regulation in the mild hyperthermia group with control, but the expression was downregulated in the other groups, especially in severe hyperthermia the expression was significantly downregulated (p<0.5).
IL-6 was upregulated highly and significantly in the group of berberine and all groups of combinations, whereas mild and moderate hyperthermia stimulated significant expression of IL-11 mRNA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nashiri, Muhammad& al-Khatib, Alya& Froemming, Gabriele. 2018. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences،Vol. 12, no. 1, pp.37-43.
https://search.emarefa.net/detail/BIM-897745
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nashiri, Muhammad…[et al.]. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences Vol. 12, no. 1 (2018), pp.37-43.
https://search.emarefa.net/detail/BIM-897745
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nashiri, Muhammad& al-Khatib, Alya& Froemming, Gabriele. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences. 2018. Vol. 12, no. 1, pp.37-43.
https://search.emarefa.net/detail/BIM-897745
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 42-43
رقم السجل
BIM-897745
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر