Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells
Joint Authors
Nashiri, Muhammad
al-Khatib, Alya
Froemming, Gabriele
Source
Yemeni Journal for Medical Sciences
Issue
Vol. 12, Issue 1 (31 Dec. 2018), pp.37-43, 7 p.
Publisher
University of Science and Technology
Publication Date
2018-12-31
Country of Publication
Yemen
No. of Pages
7
Main Subjects
Topics
Abstract EN
Objectives: The aim of the current study was to investigate the effects of berberine chloride and various heat conditions on the gene expression of Osterix, RUNX-2, RANKL, CDK2, CDK4, IL-6 and IL-11.
Methods: Six groups of cells were treated with hyperthermia for 1 h: Two groups at mild, two at moderate and two at severe hyperthermia (39, 43 and 45°C respectively).
Berberine chloride (80 µg/mL) was selected for treating one group of mild, moderate and severe hyperthermia (combination).
All treated groups were recovered at 37°C for 24 h.
Another exposure for hyperthermia (1 h) and recovery for 3 h at 37°C were applied.
Results: The expression of Osterix was highly upregulated in all groups except in the severe hyperthermia and mild hyperthermia with berberine groups.
Only the mild hyperthermia without berberine induced a slight increase in the expression of RUNX2, whereas severe hyperthermia alone suppressed the levels on a significant manner.
Berberine alone was more effective in significantly up-regulating RANKL expression.
On the other hand, CDK2 mRNA was downregulated in all groups.
CDK4 showed a similar regulation in the mild hyperthermia group with control, but the expression was downregulated in the other groups, especially in severe hyperthermia the expression was significantly downregulated (p<0.5).
IL-6 was upregulated highly and significantly in the group of berberine and all groups of combinations, whereas mild and moderate hyperthermia stimulated significant expression of IL-11 mRNA.
American Psychological Association (APA)
Nashiri, Muhammad& al-Khatib, Alya& Froemming, Gabriele. 2018. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences،Vol. 12, no. 1, pp.37-43.
https://search.emarefa.net/detail/BIM-897745
Modern Language Association (MLA)
Nashiri, Muhammad…[et al.]. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences Vol. 12, no. 1 (2018), pp.37-43.
https://search.emarefa.net/detail/BIM-897745
American Medical Association (AMA)
Nashiri, Muhammad& al-Khatib, Alya& Froemming, Gabriele. Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells. Yemeni Journal for Medical Sciences. 2018. Vol. 12, no. 1, pp.37-43.
https://search.emarefa.net/detail/BIM-897745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 42-43
Record ID
BIM-897745