Indirect spectrophotometric determination of niclosamide in pharmaceutical preparations

الملخص EN

A simple and sensitive indirect spectrophotometric method is developed for the determination of niclosamide in pharmaceutical preparations.

The method is based on the oxidation of reduced form of niclosamide with iron ( III ) in acidic medium, then the subsequent reaction of iron ( II ) with 1,10- phenanthroline to produce a ferroin complex which is water - soluble , stable (using EDTA solution improve the stability of the complex) , and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 2.19×104 l.mol-1 .cm -1 .

Beer,s law is valid over the concentration range of 2.5 to 225 µg/10 ml.

The relative error is in between - 1.80 and +1.50 % and the relative standard deviation from ± 0.89 to ± 2.07 % depending on the concentration of niclosamide .

The proposed method has been applied successfully to determine niclosamide in pharmaceutical preparations.