Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood

الملخص EN

The study included the collection of umbilical cords blood samples immediately after delivery .

Hematopoietic stem cells were isolated from the umbilical cord blood by the gradient density method.

The hematopoietic stem cells (MNCs) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10 % of fetal calf serum, then subjected to in vitro study to confirm the toxic effects of Trichothecenss extracts with the following concentrations (0.001,0.0039,0.0078, 0.0156, 0.031, 0.062, 0.13, 0.25, and 1 μg/ ml) and to detect the inhibition rate of hematopoietic stem cells by using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide.

The cytotoxicity assay (in vitro study), revealed that the inhibition rate increases seriously with the increase of Trichothecens concentration.

Hence the concentration 1 μg /ml has given inhibition rate which reached 100%, while the concentration 0.001 μg /ml did not eliminates any type of cells.

Trichothecens has a highly toxic effect on human hematopoietic stem cells, and its concentration is high and positively associated with the rate of cell death increases.

Furthermore, the concentration 1 μg /ml of Trichothecens extracted from the Trichothecensgenic isolates can kill 100% of hematopoietic stem cell.