Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood
Joint Authors
al-Kubaysi, Salah Mahmud Ashur
al-Hamdani, Hibat Allah Adil Abd Allah
al-Anni, Muhammad Q.
Source
Iraqi Journal of Biotechnology
Issue
Vol. 17, Issue 3 (31 Dec. 2018), pp.100-111, 12 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
The study included the collection of umbilical cords blood samples immediately after delivery .
Hematopoietic stem cells were isolated from the umbilical cord blood by the gradient density method.
The hematopoietic stem cells (MNCs) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10 % of fetal calf serum, then subjected to in vitro study to confirm the toxic effects of Trichothecenss extracts with the following concentrations (0.001,0.0039,0.0078, 0.0156, 0.031, 0.062, 0.13, 0.25, and 1 μg/ ml) and to detect the inhibition rate of hematopoietic stem cells by using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide.
The cytotoxicity assay (in vitro study), revealed that the inhibition rate increases seriously with the increase of Trichothecens concentration.
Hence the concentration 1 μg /ml has given inhibition rate which reached 100%, while the concentration 0.001 μg /ml did not eliminates any type of cells.
Trichothecens has a highly toxic effect on human hematopoietic stem cells, and its concentration is high and positively associated with the rate of cell death increases.
Furthermore, the concentration 1 μg /ml of Trichothecens extracted from the Trichothecensgenic isolates can kill 100% of hematopoietic stem cell.
American Psychological Association (APA)
al-Hamdani, Hibat Allah Adil Abd Allah& al-Anni, Muhammad Q.& al-Kubaysi, Salah Mahmud Ashur. 2018. Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood. Iraqi Journal of Biotechnology،Vol. 17, no. 3, pp.100-111.
https://search.emarefa.net/detail/BIM-900053
Modern Language Association (MLA)
al-Hamdani, Hibat Allah Adil Abd Allah…[et al.]. Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood. Iraqi Journal of Biotechnology Vol. 17, no. 3 (Dec. 2018), pp.100-111.
https://search.emarefa.net/detail/BIM-900053
American Medical Association (AMA)
al-Hamdani, Hibat Allah Adil Abd Allah& al-Anni, Muhammad Q.& al-Kubaysi, Salah Mahmud Ashur. Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood. Iraqi Journal of Biotechnology. 2018. Vol. 17, no. 3, pp.100-111.
https://search.emarefa.net/detail/BIM-900053
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 109-111
Record ID
BIM-900053