Anti-cancer activity of a novel palladium (II)‎ complex against cervical, breast and colorectal cancer cell lines in vitro

العناوين الأخرى

كفاءة مركب مشتق من معدن البالاديوم ضد خلايا سرطان عنق الرحم و الثدي و القولون معمليا

مقدم أطروحة جامعية

Ramadan, Nura Zuhayr Khamis

مشرف أطروحة جامعية

al-Bashiti, Tariq Abd al-Qadir
al-Uwayni, Saib Husayn

الجامعة

الجامعة الإسلامية

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

فلسطين (قطاع غزة)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2017

الملخص الإنجليزي

Introduction: Cancer is the main cause of morbidity and mortality worldwide, with 14 million new cases in 2012; it is expected to rise 70% during the next 2 decades.

In 2015, it was responsible for 8.8 million deaths; nearly one in six deaths is due to cancer.

In 2010, the economic cost of cancer was estimated US$ 1.16 trillion annually.

Problem: Platinum based drugs such as cisplatin ([cis-PtCl2(NH3)2]) are the most effective and widely used as platinum-metal chemotherapy anticancer drug, but cisplatin has significant disadvantages including poor solubility, inducing tumor cell resistance, and multiple serious side effects.

Objectives: This research was conducted to evaluate anticancer activity of newly synthesized a palladcyclometallated complex (C79H68Cl2N2O9P2Pd2) named ASH10.

This includes its effect on breast, cervical and colorectal cell lines.

Furthermore, this project provides some indications about the mechanism of action of ASH10.

Methodology: The cytotoxic and anti-proliferative effects of dinuclear palladium complex were determined in MCF7, HeLa and CACO-2 cell lines by MTT assay, trypan blue, scratch assay and western blot to evaluate the mechanism induced by ASH10.

Results: Our results showed that the palladium complex has strong anti-growth effect in time and concentration dependent manner in vitro against all cancer cell lines used in the study.

The cytotoxic effect of ASH10 against cervical cancer cell line was potent with an IC50 values of 6.9 µM and similar effect was shown on MCF7 as 40% viability recorded at 8 µM.

In addition, ASH10 induced significant cytotoxic effect against colorectal cancer cells with an IC50 of 12.1 µM.

The mechanism by which ASH10 inhibits cancer cells might be apoptosis as evident by the increasing level of poly ADP ribose polymerase (PARP) cleavage after ASH10 treatment on Hela cells.

Conclusions: This newly synthesized palladium II complex (ASH10) represents a potential active novel anticancer drug against cervical, breast and colorectal human tumors in vitro.

التخصصات الرئيسية

الأحياء

عدد الصفحات

74

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-900927