Anti-cancer activity of a novel palladium (II) complex against cervical, breast and colorectal cancer cell lines in vitro
Other Title(s)
كفاءة مركب مشتق من معدن البالاديوم ضد خلايا سرطان عنق الرحم و الثدي و القولون معمليا
Dissertant
Thesis advisor
al-Bashiti, Tariq Abd al-Qadir
al-Uwayni, Saib Husayn
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2017
English Abstract
Introduction: Cancer is the main cause of morbidity and mortality worldwide, with 14 million new cases in 2012; it is expected to rise 70% during the next 2 decades.
In 2015, it was responsible for 8.8 million deaths; nearly one in six deaths is due to cancer.
In 2010, the economic cost of cancer was estimated US$ 1.16 trillion annually.
Problem: Platinum based drugs such as cisplatin ([cis-PtCl2(NH3)2]) are the most effective and widely used as platinum-metal chemotherapy anticancer drug, but cisplatin has significant disadvantages including poor solubility, inducing tumor cell resistance, and multiple serious side effects.
Objectives: This research was conducted to evaluate anticancer activity of newly synthesized a palladcyclometallated complex (C79H68Cl2N2O9P2Pd2) named ASH10.
This includes its effect on breast, cervical and colorectal cell lines.
Furthermore, this project provides some indications about the mechanism of action of ASH10.
Methodology: The cytotoxic and anti-proliferative effects of dinuclear palladium complex were determined in MCF7, HeLa and CACO-2 cell lines by MTT assay, trypan blue, scratch assay and western blot to evaluate the mechanism induced by ASH10.
Results: Our results showed that the palladium complex has strong anti-growth effect in time and concentration dependent manner in vitro against all cancer cell lines used in the study.
The cytotoxic effect of ASH10 against cervical cancer cell line was potent with an IC50 values of 6.9 µM and similar effect was shown on MCF7 as 40% viability recorded at 8 µM.
In addition, ASH10 induced significant cytotoxic effect against colorectal cancer cells with an IC50 of 12.1 µM.
The mechanism by which ASH10 inhibits cancer cells might be apoptosis as evident by the increasing level of poly ADP ribose polymerase (PARP) cleavage after ASH10 treatment on Hela cells.
Conclusions: This newly synthesized palladium II complex (ASH10) represents a potential active novel anticancer drug against cervical, breast and colorectal human tumors in vitro.
Main Subjects
No. of Pages
74
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusions and recommendations.
References.
American Psychological Association (APA)
Ramadan, Nura Zuhayr Khamis. (2017). Anti-cancer activity of a novel palladium (II) complex against cervical, breast and colorectal cancer cell lines in vitro. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-900927
Modern Language Association (MLA)
Ramadan, Nura Zuhayr Khamis. Anti-cancer activity of a novel palladium (II) complex against cervical, breast and colorectal cancer cell lines in vitro. (Master's theses Theses and Dissertations Master). Islamic University. (2017).
https://search.emarefa.net/detail/BIM-900927
American Medical Association (AMA)
Ramadan, Nura Zuhayr Khamis. (2017). Anti-cancer activity of a novel palladium (II) complex against cervical, breast and colorectal cancer cell lines in vitro. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-900927
Language
English
Data Type
Arab Theses
Record ID
BIM-900927
