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The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm
العناوين الأخرى
تأثير فترة الحفظ على جودة، مستوى اكسدة الدهون، و التلف في الحمض النووي ما بعد الإذابة لحيامن ثور ماشية بالي
المؤلفون المشاركون
Duinovanto, Huda
Rimayanti
Mustafa, Imam
Susilowati, Sahirni
Hernawati, Tatic
المصدر
Iraqi Journal of Veterinary Sciences
العدد
المجلد 32، العدد 2 (31 ديسمبر/كانون الأول 2018)، ص ص. 249-252، 4ص.
الناشر
جامعة الموصل كلية الطب البيطري
تاريخ النشر
2018-12-31
دولة النشر
العراق
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
the steps of semen freezing to obtain high fertility sperm after added with the extender.
This study was aimed to determined the sperm quality (motility, viability, and membrane integrity), MDA level and DNA damage of post-thawed sperm after being preserved at 5ºC for different duration.
Bali cattle bull semen collected by artificial vagina.
Macroscopic and microscopic evaluation of ejaculate was conducted first.
Qualified semen was diluted in Tris Aminomethan-Egg yolk and then devided into two treatment group: preserved at 5ºC for 4 hours (first group) and 22 hours (second group), and continued to processed into frozen semen, and stored overnight.
Pre-freezing and post-thawed of semen analysis was conducted based on SNI: 4869- 1:2017 of The National Standardization Agency of Indonesia.
Measurement of levels of Malondialdehyde (MDA) was conducted by spectrophotometry at 532 nm wavelength, meanwhile measurement of sperms DNA damage percentage was performed by Toluidine blue staining.
The result of showed that quality of post-thawed sperm (motility, viability, and membrane integrity) was higher (P < 0.05), and MDA level and DNA damage were lower (P < 0.05) in preservation at 5°C for 22 hours compared to those of 4 hours.
It could be concluded that preservation at 5°C longer (22 hours) means sperm had longer chance to adapt with the extender, this implies the higher quality and lower lipid peroxidation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duinovanto, Huda& Rimayanti& Susilowati, Sahirni& Mustafa, Imam& Hernawati, Tatic. 2018. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 2, pp.249-252.
https://search.emarefa.net/detail/BIM-900981
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duinovanto, Huda…[et al.]. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences Vol. 32, no. 2 (2018), pp.249-252.
https://search.emarefa.net/detail/BIM-900981
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duinovanto, Huda& Rimayanti& Susilowati, Sahirni& Mustafa, Imam& Hernawati, Tatic. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 2, pp.249-252.
https://search.emarefa.net/detail/BIM-900981
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 252
رقم السجل
BIM-900981
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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