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The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm
Other Title(s)
تأثير فترة الحفظ على جودة، مستوى اكسدة الدهون، و التلف في الحمض النووي ما بعد الإذابة لحيامن ثور ماشية بالي
Joint Authors
Duinovanto, Huda
Rimayanti
Mustafa, Imam
Susilowati, Sahirni
Hernawati, Tatic
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 32, Issue 2 (31 Dec. 2018), pp.249-252, 4 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Abstract EN
the steps of semen freezing to obtain high fertility sperm after added with the extender.
This study was aimed to determined the sperm quality (motility, viability, and membrane integrity), MDA level and DNA damage of post-thawed sperm after being preserved at 5ºC for different duration.
Bali cattle bull semen collected by artificial vagina.
Macroscopic and microscopic evaluation of ejaculate was conducted first.
Qualified semen was diluted in Tris Aminomethan-Egg yolk and then devided into two treatment group: preserved at 5ºC for 4 hours (first group) and 22 hours (second group), and continued to processed into frozen semen, and stored overnight.
Pre-freezing and post-thawed of semen analysis was conducted based on SNI: 4869- 1:2017 of The National Standardization Agency of Indonesia.
Measurement of levels of Malondialdehyde (MDA) was conducted by spectrophotometry at 532 nm wavelength, meanwhile measurement of sperms DNA damage percentage was performed by Toluidine blue staining.
The result of showed that quality of post-thawed sperm (motility, viability, and membrane integrity) was higher (P < 0.05), and MDA level and DNA damage were lower (P < 0.05) in preservation at 5°C for 22 hours compared to those of 4 hours.
It could be concluded that preservation at 5°C longer (22 hours) means sperm had longer chance to adapt with the extender, this implies the higher quality and lower lipid peroxidation.
American Psychological Association (APA)
Duinovanto, Huda& Rimayanti& Susilowati, Sahirni& Mustafa, Imam& Hernawati, Tatic. 2018. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 2, pp.249-252.
https://search.emarefa.net/detail/BIM-900981
Modern Language Association (MLA)
Duinovanto, Huda…[et al.]. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences Vol. 32, no. 2 (2018), pp.249-252.
https://search.emarefa.net/detail/BIM-900981
American Medical Association (AMA)
Duinovanto, Huda& Rimayanti& Susilowati, Sahirni& Mustafa, Imam& Hernawati, Tatic. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 2, pp.249-252.
https://search.emarefa.net/detail/BIM-900981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 252
Record ID
BIM-900981