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Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources
المؤلف
المصدر
ZANCO Journal of Pure and Applied Sciences
العدد
المجلد 30، العدد 5 (31 أكتوبر/تشرين الأول 2018)، ص ص. 101-113، 13ص.
الناشر
جامعة صلاح الدين قسم النشر العلمي
تاريخ النشر
2018-10-31
دولة النشر
العراق
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Staphylococcus hominis is an opportunistic gram positive bacteria and is a member of coagulase-negative staphylococci (CoNS) .
During the study, 213 clinical specimens were collected from patients admitted to different hospitals in Erbil city, Iraq.
Only 18(8.45%) isolates of S.
hominis were isolated including 7 isolates (17.07%) from blood, 4 isolates (8.70%) from urine, 3 isolates (7.14%) from ear, 2 isolates (6.67%) from wound, and one isolate from each nasal swab (4.55%) and oral cavity (3.13%), all S.
hominis identified based on morphology, cultural, biochemical tests, and further confirmed by Vitek 2 compact system.
To determine the most accurate assay for measuring methicillin resistance S.
hominis, compared the detection of mecA by PCR with detection by National Committee for Clinical Laboratory Standards methods using oxacillin and Cefoxitin disk diffusion method.
The results of oxacillin showed 13 (72.22%) isolates resistant to methicillin and 5 (27.77%) isolates were sensitive to it.
While, the results of cefoxitin demonstrated that 16 (88.89%) isolates were resistant to methicillin and only 2 isolates (11.11%) were sensitive to it.
However, the same results of the Cefoxitin disk diffusion method was obtained by PCR and by using mecA gene which 16 isolates (88.89%) were carried mecA gene with product size 499bp.
The results of microtiter plate method revealed that 16 (88.89%) isolates of S.
hominis were biofilm producer and only 2 isolates (11.11%) were non-biofilm producer.
Moreover,of 16 biofilm producer isolates, 14 isolates (77.77%) were categorized as strong biofilm producers and 2 (11.11%) isolates were identified as moderate biofilm producers
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mustafa, Khadijah Khalil. 2018. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences،Vol. 30, no. 5, pp.101-113.
https://search.emarefa.net/detail/BIM-901616
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mustafa, Khadijah Khalil. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences Vol. 30, no. 5 (2018), pp.101-113.
https://search.emarefa.net/detail/BIM-901616
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mustafa, Khadijah Khalil. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences. 2018. Vol. 30, no. 5, pp.101-113.
https://search.emarefa.net/detail/BIM-901616
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 110-113
رقم السجل
BIM-901616
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