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Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources
Author
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 30, Issue 5 (31 Oct. 2018), pp.101-113, 13 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2018-10-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
Staphylococcus hominis is an opportunistic gram positive bacteria and is a member of coagulase-negative staphylococci (CoNS) .
During the study, 213 clinical specimens were collected from patients admitted to different hospitals in Erbil city, Iraq.
Only 18(8.45%) isolates of S.
hominis were isolated including 7 isolates (17.07%) from blood, 4 isolates (8.70%) from urine, 3 isolates (7.14%) from ear, 2 isolates (6.67%) from wound, and one isolate from each nasal swab (4.55%) and oral cavity (3.13%), all S.
hominis identified based on morphology, cultural, biochemical tests, and further confirmed by Vitek 2 compact system.
To determine the most accurate assay for measuring methicillin resistance S.
hominis, compared the detection of mecA by PCR with detection by National Committee for Clinical Laboratory Standards methods using oxacillin and Cefoxitin disk diffusion method.
The results of oxacillin showed 13 (72.22%) isolates resistant to methicillin and 5 (27.77%) isolates were sensitive to it.
While, the results of cefoxitin demonstrated that 16 (88.89%) isolates were resistant to methicillin and only 2 isolates (11.11%) were sensitive to it.
However, the same results of the Cefoxitin disk diffusion method was obtained by PCR and by using mecA gene which 16 isolates (88.89%) were carried mecA gene with product size 499bp.
The results of microtiter plate method revealed that 16 (88.89%) isolates of S.
hominis were biofilm producer and only 2 isolates (11.11%) were non-biofilm producer.
Moreover,of 16 biofilm producer isolates, 14 isolates (77.77%) were categorized as strong biofilm producers and 2 (11.11%) isolates were identified as moderate biofilm producers
American Psychological Association (APA)
Mustafa, Khadijah Khalil. 2018. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences،Vol. 30, no. 5, pp.101-113.
https://search.emarefa.net/detail/BIM-901616
Modern Language Association (MLA)
Mustafa, Khadijah Khalil. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences Vol. 30, no. 5 (2018), pp.101-113.
https://search.emarefa.net/detail/BIM-901616
American Medical Association (AMA)
Mustafa, Khadijah Khalil. Detection of biofilm producer methicillin resistant staphylococcus hominis isolated from different clinical sources. ZANCO Journal of Pure and Applied Sciences. 2018. Vol. 30, no. 5, pp.101-113.
https://search.emarefa.net/detail/BIM-901616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 110-113
Record ID
BIM-901616