Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system
المؤلفون المشاركون
Ghramh, Hamid Ali
Kilani, Muna
Taha, Ramadan M.
Ibrahim, Isam H.
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 7 (30 نوفمبر/تشرين الثاني 2019)، ص ص. 1676-1681، 6ص.
الناشر
تاريخ النشر
2019-11-30
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
One of the mosquito-borne zoonotic diseases is the Rift Valley fever virus (RVFV).
Currently, there is no completely licensed vaccine that can be used to vaccinate animals or humans outside endemic areas.
The aim of this work was to use the RVFV glycoprotein (Gn) and the subunit B of cholera toxin (CTB) at gene level and build up fused recombinant vaccine.
The gene of CTB was joined to the gene Gn to work as an adjuvant in the resulting fusion protein.
The designed merged genes (CTB-Gn) was tested for restriction sites, open reading frames, expected fusion protein tertiary structure and antigenicity using computer software.
The insert sequence was submitted to the BioProject (GenBank).
The insert was subcloned into the pQE-31 expression plasmid.
The target recombinant protein (rCTB-Gn) was expressed in M15 bacteria, purified and identified by protein gel electrophoresis.
The insert got the accession No: PRJNA386723.
Analysis of the designed rCTB-Gn protein revealed that it had the right 3D structure, immunogenic and at the correct molecular weight.
The presence of the CTB in the proposed vaccine will augment its immunogenicity.
Doses and protection levels of the vaccine need to be manipulated.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ibrahim, Isam H.& Taha, Ramadan M.& Ghramh, Hamid Ali& Kilani, Muna. 2019. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Taha, Ramadan M.…[et al.]. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ibrahim, Isam H.& Taha, Ramadan M.& Ghramh, Hamid Ali& Kilani, Muna. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1680-1681
رقم السجل
BIM-910293
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر