Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system
Joint Authors
Ghramh, Hamid Ali
Kilani, Muna
Taha, Ramadan M.
Ibrahim, Isam H.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1676-1681, 6 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
One of the mosquito-borne zoonotic diseases is the Rift Valley fever virus (RVFV).
Currently, there is no completely licensed vaccine that can be used to vaccinate animals or humans outside endemic areas.
The aim of this work was to use the RVFV glycoprotein (Gn) and the subunit B of cholera toxin (CTB) at gene level and build up fused recombinant vaccine.
The gene of CTB was joined to the gene Gn to work as an adjuvant in the resulting fusion protein.
The designed merged genes (CTB-Gn) was tested for restriction sites, open reading frames, expected fusion protein tertiary structure and antigenicity using computer software.
The insert sequence was submitted to the BioProject (GenBank).
The insert was subcloned into the pQE-31 expression plasmid.
The target recombinant protein (rCTB-Gn) was expressed in M15 bacteria, purified and identified by protein gel electrophoresis.
The insert got the accession No: PRJNA386723.
Analysis of the designed rCTB-Gn protein revealed that it had the right 3D structure, immunogenic and at the correct molecular weight.
The presence of the CTB in the proposed vaccine will augment its immunogenicity.
Doses and protection levels of the vaccine need to be manipulated.
American Psychological Association (APA)
Ibrahim, Isam H.& Taha, Ramadan M.& Ghramh, Hamid Ali& Kilani, Muna. 2019. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
Modern Language Association (MLA)
Taha, Ramadan M.…[et al.]. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
American Medical Association (AMA)
Ibrahim, Isam H.& Taha, Ramadan M.& Ghramh, Hamid Ali& Kilani, Muna. Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1676-1681.
https://search.emarefa.net/detail/BIM-910293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1680-1681
Record ID
BIM-910293