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Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia
المؤلف
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 27، العدد 1 (31 يناير/كانون الثاني 2020)، ص ص. 222-228، 7ص.
الناشر
تاريخ النشر
2020-01-31
دولة النشر
السعودية
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
الملخص EN
Tomato is known as a highly valuable crop and grown worldwide for various uses.
The cultivation and tomato production severely affected globally by several diseases caused by various pathogens.
Begomoviruses causes yellow mosaic and leaf curl disease of tomato in the tropical, subtropical, temperate, and semi-arid regions.
In Saudi Arabia, the tomato production adversely affected by disease caused by begomoviruses known as TYLCV and ToLCSDV.
In this study, the pathogen was identified by Polymerase Chain Reaction using virus-specific primers and transmitted by whiteflies to healthy tomato seedlings.
In a field survey, the tomato plants were exhibiting symptoms like viral infection.
The infected leaf was randomly collected from various fields of tomato growing areas like Jeddah, Makkah, Tabuk, and Hail.
The full-length viral genome was amplified by Rolling Circle Amplification technology (RCA) while betasatellites were amplified by PCR using universal betasatellites primers.
The full-length viral genome (∼2.7 kb) and betasatellites (∼1.4 kb) were cloned and sequenced bi-directionally.
The generated sequences were assembled and analyzed to find out the genetic variability by using bioinformatics tools and the genetic variability and phylogenetic relationships with selected begomoviruses were analyzed.
The sequences showed the highest identity with an isolate of ToLCSDV and TYLCV.
The nucleotide similarity and phylogenetic relationship showed the closest cluster with ToLCSDV and TYLCV.
The data generated in this study elucidate that the causal organism is a variant of either TYLCV or ToLCSDV.
The provided information from this study will be highly valuable for researchers and vegetable growers not only in Saudi Arabia but also in Arabian Peninsula.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sohrab, Sayyid Sartaj. 2020. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.222-228.
https://search.emarefa.net/detail/BIM-920572
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sohrab, Sayyid Sartaj. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.222-228.
https://search.emarefa.net/detail/BIM-920572
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sohrab, Sayyid Sartaj. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.222-228.
https://search.emarefa.net/detail/BIM-920572
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 227-228
رقم السجل
BIM-920572
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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