Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”
المؤلفون المشاركون
al-Attar, Abd al-Rahman A.
Abd al-Salam, Islam M.
Qahtan, Ahmad A.
Faysal, Muhammad
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 27، العدد 1 (31 يناير/كانون الثاني 2020)، ص ص. 412-416، 5ص.
الناشر
تاريخ النشر
2020-01-31
دولة النشر
السعودية
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
There are more than nine thousand cultivars of Hibiscus rosa-sinensis L., with a series of flowers with shapes, colors and new cultivars continues as generated through both traditional and modern breeding techniques.
In this study, advanced biotech methods of in vitro culture have been used to identify a technique for the efficient mass multiplication of H.
rosa-sinensis ‘White Butterfly’, using phenyl urea, N-(2-Chloro-4-pyridyl)-N′-phenylurea (4-CPPU).
For the first time, the effects of 4-CPPU for stimulating axillary shoot proliferation and multiple shoot regenerations from nodal explants were evaluated, and the optimal nutrient media deduced.
From the diverse concentrations as 0.1, 0.5, 2.5, 5.0 & 10.0 µM of 4-CPPU, the highest frequency of shoots was recorded at 2.5 µM supplied in Murashige and Skoog (MS, pH-5.8) medium.
After eight-weeks of culture, on an average of 6.7 shoot were obtained on this media with shoot heights of 4.2 cm from each explant.
With the involvement of 0.5 µM-IBA (indole-3-butyric acid) in MS medium the regenerated shoots were rooted and followed by successful acclimation to ex vitro conditions.
The ploidy consistency among the micro-plants was analyzed using flow cytometry and compared with ex vitro grown plants.
No differences in the ploidy levels were observed among the 4-CPPU induced plants, when compared with the donor plants.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faysal, Muhammad& al-Attar, Abd al-Rahman A.& Abd al-Salam, Islam M.& Qahtan, Ahmad A.. 2020. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.412-416.
https://search.emarefa.net/detail/BIM-920704
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faysal, Muhammad…[et al.]. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.412-416.
https://search.emarefa.net/detail/BIM-920704
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faysal, Muhammad& al-Attar, Abd al-Rahman A.& Abd al-Salam, Islam M.& Qahtan, Ahmad A.. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.412-416.
https://search.emarefa.net/detail/BIM-920704
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 415-416
رقم السجل
BIM-920704
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر